玉米须绞股蓝复合提取总黄酮工艺及其降压片的制备
Extraction process of total flavonoids from stigma maydis and Gynostemma pentaphyllum and preparation of the antihypertensive tablets
刘霞 1马泽刚 2马林3
作者信息
- 1. 四川化工职业技术学院,四川 泸州 646000
- 2. 四川卫生康复职业学院药学系,四川 自贡 643000
- 3. 西南科技大学生命科学与工程学院,四川 绵阳 621002
- 折叠
摘要
以玉米须(stigma maydis)、绞股蓝(Gynostemma pentaphyllum)(1∶1)为提取原料,总黄酮含量为考察指标,通过提取溶剂、乙醇体积分数、提取温度、提取时间、料液比和提取次数等单因素考察对复合总黄酮含量的影响,再通过正交试验优化总黄酮的提取工艺,最后将优化提取的总黄酮过D101大孔吸附树脂精制后制备成降压片.结果表明,复方总黄酮的最佳提取工艺为乙醇体积分数50%,料液比1∶10,50℃下提取3.5 h.制备的片剂质量(片重差异±5.1%,脆碎度0.83%,崩解时限2.41 min)符合《中华人民共和国药典》(2020版)片剂的有关规定,并且降压片能降低SHR大鼠血压.
Abstract
Taking stigma maydis and Gynostemma pentaphyllum(1∶1)as the extraction raw material and the total flavonoids content as the investigation index,the effect of compound total flavonoids content was investigated by single factors including extraction sol-vent,ethanol volume fraction,extraction temperature,extraction time,material-liquid ratio and extraction times.Then,the extrac-tion process of total flavonoids was optimized by the orthogonal test.Finally,the optimized total flavonoids were refined by D101 mac-roporous adsorption resin and prepared into antihypertensive tablets.The results showed that the best extraction process of compound total flavonoids was 50%ethanol volume fraction,the material-liquid ratio was 1∶10,and the extraction time was 3.5 h at 50℃.The quality of the prepared tablets(weight difference±5.1%,fragmentability 0.83%,disintegration time 2.41 min)was in line with the rel-evant provisions of the Pharmacopoeia of the People's Republic of China(2020 edition),and the antihypertensive tablets could re-duce the blood pressure of SHR rats.
关键词
玉米须(stigma/maydis)/绞股蓝(Gynostemma/pentaphyllum)/总黄酮/提取工艺/降压片/制备Key words
stigma maydis/Gynostemma pentaphyllum/total flavonooids/extraction process/antihypertensive tablets/preparation引用本文复制引用
基金项目
四川省教育厅自然科学研究项目(17ZB0366)
四川化工职业技术学院重点课题(SCHYA-1904)
出版年
2024
国家科技期刊平台
NSTL
万方数据