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壮药广西鹅掌柴质量标准建立

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采用显微鉴别和薄层色谱鉴别方法对不同产地壮药广西鹅掌柴进行鉴定研究,按照《中华人民共和国药典》2020年版四部通则中规定的方法对不同产地药材进行水分含量、灰分含量和浸出物含量的测定。结果表明,壮药广西鹅掌柴显微鉴别特征性强;薄层色谱鉴别方法以环己烷∶丙酮∶甲酸(8。5∶1。5∶0。4)为展开系统,此展开系统分离度较好,斑点清晰,以15 cm为展距,斑点分离度最佳;以10%硫酸乙醇溶液作为显色剂,紫外灯(365 nm)下检视;拟定壮药广西鹅掌柴水分含量不超过12。00%,总灰分含量不超过6。00%,酸不溶性灰分含量不超过0。50%,水溶性浸出物含量不低于9。50%。其方法操作简单易行、稳定可靠、重复性较好、拟定范围合理,可为壮药广西鹅掌柴质量控制提供理论依据。
Establishment of quality standards for Zhuang medicine Schefflera kwangsiensis
Microscopic identification and thin-layer chromatography identification methods were used to identify and study the Zhuang medicine Schefflera kwangsiensis from different regions.The moisture content,ash content,and extract content of the medicinal materi-als from different regions were determined according to the methods specified in the 2020 edition of the"Pharmacopoeia of the People's Republic of China".The results showed that the microscopic identification characteristics of the Zhuang medicine Schefflera kwangsien-sis were strong;the thin-layer chromatography identification method used cyclohexane∶acetone∶formic acid of 8.5∶1.5∶0.4 as the un-folding system,which had good separation and clear spots.The best spot separation was achieved with a spreading distance of 15 cm;using a 10%sulfuric acid ethanol solution as the chromogenic agent,the examination was conducted under a UV lamp(365 nm);the moisture content of Zhuang medicine Schefflera kwangsiensis shall not exceed 12.00%,the total ash content should not exceed 6.00%,the acid insoluble ash content should not exceed 0.50%,and the water-soluble extract content should not be less than 9.50%.The method was easy to operate,stable and reliable,with good reproducibility and reasonable scope,which could provide a theoretical ba-sis for the quality control of Zhuang medicine Schefflera kwangsiensis.

Zhuang medicineSchefflera kwangsiensisquality standardsthin-layer chromatography

温海成、苏永精、谢澳、李灿、韦威

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广西中医药大学壮医药学院,南宁 530200

壮药 广西鹅掌柴 质量标准 薄层色谱

广西科技厅重点研发项目广西中药药效研究重点实验室项目

桂科AB1911002720-065-38

2024

湖北农业科学
湖北省农业科学院 华中农业大学 长江大学 黄冈师范学院

湖北农业科学

CSTPCD
影响因子:0.442
ISSN:0439-8114
年,卷(期):2024.63(4)
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