Effect of medium cost reduction measures on the growth of sweet potato tissue culture seedlings under continuous subculture
In order to reduce the production cost of rapid propagation of sweet potato(Dioscorea esculenta(Lour.)Burgill)tissue cul-ture seedlings and improve the economic benefits of factory seedling cultivation,on the basis of MS basic culture medium,the effects of simplifying or replacing the composition of culture medium on the growth of sweet potato tissue culture seedlings under continuous subculture were studied through cost reduction measures such as simplifying the composition of culture medium,replacing deionized water with tap water,and replacing sucrose with granulated sugar.The results showed that using white sugar instead of sucrose had no significant effect on the growth of sweet potato tissue cultured seedlings,whether in primary or secondary culture.However,removing trace elements and organic matter from the culture medium,or using tap water instead of deionized water,all had adverse effects on tissue culture seedlings,leading to a decrease in plant height,a decrease in proliferation coefficient,and poorer root growth,and this adverse effect was further exacerbated after prolonged subculture,resulting in poorer growth of tissue cultured seedlings.Therefore,in the factory production of sweet potato tissue culture seedlings,white sugar could be used instead of sucrose to reduce the production cost of tissue culture,while other cost reduction measures were not suitable.
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