首页|壮药蛇尾草HPLC指纹图谱及抗氧化活性的谱效关系

壮药蛇尾草HPLC指纹图谱及抗氧化活性的谱效关系

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为研究壮药蛇尾草高效液相(HPLC)指纹图谱及抗氧化活性间的谱效关系，建立了蛇尾草药材高效液相指纹图谱，采用1，1-二苯基-2-三硝基苯肼法(DPPH)和铁离子还原能力法(FRAP)对蛇尾草抗氧化活性进行评价，结合聚类分析、主成分分析和偏最小二乘分析对蛇尾草抗氧化活性进行谱效分析。结果表明，以3号峰(毛蕊花糖苷)为参照峰，确定了11个共有峰，大部分批次蛇尾草的相似度>0。900;通过聚类分析和主成分分析，可将22 批样品分为两类;不同产地间蛇尾草毛蕊花糖苷含量在0。38%～4。82%;22批蛇尾草抗氧化活性良好，共有峰1、2、3(毛蕊花糖苷)、6、8、10、11与DPPH自由基清除率呈正相关，1、2、3(毛蕊花糖苷)、6、10号峰与铁离子还原能力呈正相关。建立的蛇尾草HPLC指纹图谱可靠、准确，可为蛇尾草抗氧化活性物质的筛选及质量控制提供参考。
HPLC fingerprint and its spectral effect relationship with antioxidant activity of Zhuang medicine Pogonostemon auricularius(L.)Hassk
Aiming to study the relationship between high performance liquid ehromatography(HPLC)fingerprint and antioxidant ac-tivity,the HPLC fingerprint of Pogonostemon auricularius(L.)Hassk.was established.Combing with Cluster Analysis,Principal Component Analysis,and Partial Least Squares Regression Analysis,the antioxidant activity of Pogonostemon auricularius(L.)Has-sk.was evaluated by 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)and ferric reducing ability of plasma(FRAP)methods.The re-sults showed that eleven common peaks were identified with peak No.3(verbascoside)as the reference peak,and the similarity of most batches of Pogonostemon auricularius(L.)Hassk.was greater 0.900.Through cluster analysis and principal component analysis,twenty-two batches of samples could be divided into two categories.The content of verbascoside in Pogonostemon auricularius(L.)Hassk.from different producing areas ranged from 0.38%to 4.82%.The antioxidant activity of twenty-two lots of Pogonostemon auricu-larius(L.)Hassk.was good.Peaks 1,2,3(verbascoside),6,8,10,and 11 were positively correlated with DPPH free radical scav-enging rate,peaks 1,2,3(verbascoside),6,and 10 were positively correlated with iron ion reducing ability.The established HPLC fingerprint was reliable and accurate,which could provide reference for the screening and quality control of antioxidant active sub-stances of Pogonostemon auricularius(L.)Hassk.

Pogonostemon auricularius(L.)Hassk.fingerprintcluster analysisprincipal component analysisantioxidant activi-tyspectrum effect relationshipPartial Least Squares Regression Analysis

马雯芳、吴剑丽、张秀丽、蓝昌斌、甘洁雪、周华锋、王剑

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广西中医药大学药学院,南宁 530001

蛇尾草 指纹图谱 聚类分析 主成分分析 抗氧化活性 谱效关系 偏最小二乘回归分析

壮瑶药协同创新中心项目广西壮瑶药重点实验室项目广西重点学科壮药学项目广西协同创新中心壮瑶药协同创新中心科研项目

桂教科研[2013]20号桂科基字[2014]32号桂教科研[2013]16号ZYXT[2015]-13

2024

10.14088/j.cnki.issn0439-8114.2024.05.015

湖北农业科学
湖北省农业科学院　华中农业大学　长江大学　黄冈师范学院

湖北农业科学

CSTPCD
影响因子：0.442
ISSN：0439-8114
年,卷(期)：2024.63(5)
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