In order to clarify the subcellular spatial localisation of ester catechins in small and medium-leaf teas,five small-and me-dium-leaf tea samples of Shilixiang,Guilv No.1,Zhenong 113,Fuding Dabai Tea and Zhenong 139 were taken as the test materials,and the content of ester catechins in the five tea samples was detected using high-performance liquid chromatography(HPLC),and the vesicles in the cells of the samples were localised using neutral red staining.Subcellular localisation of ester catechins in tea sam-ples was performed by immunohistochemistry.The results showed that all five types of small and medium-leaf teas contained different levels of ester catechins,with the highest content in Shilixiang tea;under neutral red staining,the vesicles in Shilixiang tea samples were round or oval;under immunohistochemistry staining,the ester catechins of the five types of small and medium-leaf teas were lo-cated in the vesicles at the subcellular level.
关键词
中小种茶叶/酯型儿茶素/免疫组织化学法/亚细胞定位/含量测定
Key words
small and medium-leaf teas/ester catechins/immunohistochemistry/subcellular localisation/determination of content
维普
万方数据