Optimization of extraction process of total alkaloids from Pinellia ternata and their biological activity analysis
Taking the tuber of Pinellia ternata as raw material and ethanol as ultrasonic-assisted extraction solvent,the influence of single factors such as ethanol volume fraction,ultrasonic time,material-liquid ratio and ultrasonic temperature on total alkaloids ex-traction rate was investigated.The orthogonal test was designed to optimize the extraction process,and the in vitro antioxidant and hy-poglycemic activities of total alkaloids were tested.The results showed that the optimal extraction condition was ethanol volume fraction of 65%,extraction time of 60 min,the material-liquid ratio of 1∶45,ultrasonic temperature of 65℃,and the extraction rate of total alkaloids was 0.502%;the clearance of DPPH and ABTS radicals reached 89.90%and 43.60%,respectively,and the inhibition of α-glucosidase reached 86.40%.The process was simple and feasible,and the total alkaloids showed good in vitro antioxidant and hypo-glycemic biological activities.
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