首页|连翘总黄酮提取工艺优化及抗炎活性研究

连翘总黄酮提取工艺优化及抗炎活性研究

扫码查看
原文链接
  • 万方数据
  • 维普
通过单因素试验和响应面试验设计，评价浸泡时间、料液比、乙醇浓度、提取温度和提取时间对连翘(Forsythia suspensa)总黄酮提取率的影响，建立数学模型，得出二次回归方程，预测连翘总黄酮提取的最佳工艺条件，并对新工艺提取的连翘总黄酮进行小鼠耳廓急性炎症肿胀抑制效果评价，测试连翘总黄酮提取物的抗炎活性。结果表明，最佳工艺条件为料液比1∶20、浸泡时间30 min、提取温度58℃、乙醇浓度60%、提取时间79 min。经验证，新工艺的平均提取率为14。54%。新工艺提取的连翘总黄酮提取液处理二甲苯急性致炎的小鼠耳廓的肿胀值为(15。61±2。73)mg，低于生理盐水处理后的肿胀值(17。21±2。65)mg，体外抗炎试验表明连翘总黄酮提取物可抑制小白鼠耳廓的炎症肿胀。引入浸泡环节的连翘总黄酮提取工艺具有提取率较高、稳定可靠的特点，新工艺提取的连翘总黄酮具有一定的抗炎效果。
Research on optimization of extraction process and anti-inflammatory activity of total flavonoids from Forsythia suspensa
The effects of soaking time,solid-liquid ratio,ethanol concentration,extraction temperature and extraction time on the ex-traction rate of total flavonoids from Forsythia suspensa were evaluated by single factor experiment and response surface experiment de-sign,and a mathematical model was built,which could be used for calculating the quadratic regression equation and predicting the op-timal process of extracting total flavonoids from Forsythia suspensa.The inhibition effect of acute inflammatory swelling of mouse auri-cle with the total flavonoids of Forsythia suspensa extracted by the new process was evaluated,and the anti-inflammatory activity of the total flavonoids of Forsythia suspensa was tested.The results showed that the optimal process condition was solid-liquid ratio of 1∶20,soaking time of 30 min,extraction temperature of 58℃,ethanol concentration of 60%,and extraction time of 79 min.It had been veri-fied that the average extraction rate of the new process was 14.54%.The total flavonoids extract of Forsythia suspensa extracted by the new process had a swelling value of(15.61±2.73)mg of the mouse auricle treated with xylene acute to inflammation,which was lower than the swelling value(17.21±2.65)mg after normal saline treatment.The anti-inflammatory in vitro showed that the extract of total flavonoids from Forsythia suspensa could inhibit inflammatory swelling of the mouse auricle.The extraction process of total flavonoids from Forsythia suspensa with the introduction of soaking process had the characteristics of high extraction rate,stability and reliability,and the Forsythia suspensa total flavonoids extracted by the new process had anti-inflammatory effects.

Forsythia suspensatotal flavonoidsextraction processanti-inflammatory

延永、靳颜颜、张亦琳、程敏、周伟

展开 >

商洛学院生物医药与食品工程学院,陕西 商洛 726000

陕西秦岭特色生物资源产业技术研究院,陕西 商洛 726000

陕西香菊药业有限公司,陕西 商洛 726000

连翘(Forsythia suspensa) 总黄酮 提取工艺 抗炎

陕西省重点研发计划陕西省教育厅服务地方专项科学研究计划(2023)商洛市科技计划陕西秦岭特色生物资源产业技术研究院开放基金(2022)陕西省大学生创新创业训练计划

2024NC-YBXM-06523JC0262022-Z001923HKY033S202311396049

2024

10.14088/j.cnki.issn0439-8114.2024.05.028

湖北农业科学
湖北省农业科学院　华中农业大学　长江大学　黄冈师范学院

湖北农业科学

CSTPCD
影响因子：0.442
ISSN：0439-8114
年,卷(期)：2024.63(5)
  • 17