Simultaneous detection of 13 phenolic acids in Gynostemma pentaphyllum from Pingli,Shaanxi Province by HPLC
In order to establish a method for simultaneously detecting the content of 13 phenolic acids in Gynostemma pentaphyllum from Pingli,Shaanxi,high performance liquid chromatography(HPLC)was used.Methanol aqueous solution(80%)was used for room temperature vortex and ultrasonic extraction,the chromatographic column was Dimensions C18(4.6 mm×150 mm,5 μm,P/N:227-30011-07,C/N:18C03033),with a mobile phase of methanol(100%)and phosphoric acid aqueous solution(0.5%).The elu-tion method was gradient elution,with a detection wavelength of 280 nm,column temperature of 30℃,flow rate of 1.0 mL/min,and injection volume of 5 μL.The results showed that there was a good linear relationship among the 13 phenolic acids at concentrations of 5～100 mg/L,with a correlation coefficient(R2)≥0.998;in the sample spiking recovery experiment,the average spiking recovery rate was 61.8%～131.0%.This determination method had good precision,repeatability,and stability,and was suitable for the determi-nation of phenolic acids content in Gynostemma pentaphyllum from Pingli,Shaanxi.
Gynostemma pentaphyllum from Pingli,Shaanxiphenolic acidhigh performance liquid chromatography(HPLC)con-tent
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