摘要
以采集的 121 份野生枸杞(Lycium barbarum L.)种质为材料,用 9 对SSR引物进行扩增,采用GeneALEX、Power Marker软件进行多态性分析,利用NTSYS、STRUC-TURE软件进行遗传多样性分析、聚类分析和主成分分析.结果表明,9对SSR引物共检测到108个等位基因,等位基因数(Na)、有效等位基因数(Ne)、观测杂合度(Ho)、期望杂合度(He)、多态信息含量(PIC)、Shannon's信息指数分别为12个、4.620个、0.678、0.651、0.629、1.568;聚类结果显示121份野生枸杞种质可划分为5个亚群,群体遗传结构表明当K=5时,121个种质划分为5个亚群,来自宁夏的NXGY-02、NXGY-03 2个种质与来自内蒙古的6个种质之间的遗传距离最远,亲缘关系较远,来自青海省、新疆、甘肃省及内蒙古的其余野生枸杞种质之间存在广泛的基因交流现象;宁夏、内蒙古的野生枸杞种质具有独立起源进化的可能,而新疆、甘肃省、青海省的种质与宁夏种质亲缘关系较近,很可能属于同一起源.
Abstract
121 wild goji berry(Lycium barbarum L.)germplasms were collected as materials,9 pairs of SSR primers were used of am-plification,and polymorphism was analyzed using GeneALEX and Power Marker software.Genetic diversity analysis,cluster analy-sis,and principal component analysis were performed using NTSYS and STRUC-TURE software.The results showed that a total of 108 alleles were detected using 9 pairs of SSR primers.The number of alleles(Na),effective alleles(Ne),observed heterozygosity(Ho),expected heterozygosity(He),polymorphic information content(PIC),and Shannon's information index were 12,4.620,0.678,0.651,0.629,and 1.568,respectively;the clustering results showed that 121 wild goji berry germplasms could be divided into 5 sub-groups.The genetic structure of the population showed that when K=5,121 germplasms could be divided into 5 subgroups.The genetic distance between NXGY-02 and NXGY-03 germplasms from Ningxia and 6 germplasms from Inner Mongolia was the farthest,and the genetic relationship was relatively distant,there was extensive gene exchange among other wild goji berry germplasms from Qinghai Province,Xinjiang,Gansu Province,and Inner Mongolia;the wild goji berry germplasms in Ningxia and Inner Mongolia had the possi-bility of independent origin and evolution,while the germplasms in Xinjiang,Gansu,and Qinghai provinces were closely related to the germplasms in Ningxia and were likely to belong to the same origin.
基金项目
国家自然科学基金(32060360)
宁夏回族自治区重点研发计划(2020BBF020062021BEF02005)
维普
万方数据