The preliminary study of the diversity and radiation-resistant characteristics of culturable aerobic halophilic bacteria in the Aiding Lake
In order to reveal the diversity and radiation-resistant related characteristics of the culturable aerobic halophilic bacteria in Aiding Lake of Xinjiang,R2A with the salt fraction removed was used as the basal medium to isolate aerobic halophilic bacteria in the mudflat sediment and saline land around the Aiding Lake area in the range of 50～300 g/L NaCl concentration.The results showed that 80 strains of aerobic halophilic bacteria were obtained by 16S rRNA sequence and phylogenetic analysis.The number of culturable aer-obic halophilic bacteria and the species richness in the saline land around the lake were significantly higher than those in the mudflat sediment.Among them,moderate halophilic bacteria were not only more abundant but also rich in diversity.The isolated aerobic halo-philic bacteria belonged to 22 genera of the phyla Firmicutes,Actinobacteria and Proteobacteria.Among them,the Firmicutes(70.00%)was the dominant phylum and the Bacillus(45.00%)was the dominant genus.In addition,19 potential new species with high salinity were isolated.34 strains from the isolated 80 aerobic halophilic bacteria had a survival rate(SR)of more than 10%after UVC irradiation at 100 J/m2,and 25.00%of the halophilic radiation-resistant bacteria were Bacillus.Streptomyces sp.ALP-8 and Streptomyces sp.ASS-29 isolated from 100 g/L salt concentration were the most radiation-resistant,and SR was 67.23%and 65.73%respectively.The SR of extreme halophilic bacteria Staphylococcus sp.ASS-5(isolated from 300 g/L NaCl),Sediminibacillus sp.ALP-17(isolated from 250 g/L NaCl)and Kocuria sp.ASS-27(isolated from 250 g/L NaCl)was all above 50%.
culturable bacteriahalophilic bacteriadiversityradiation-resistantAiding Lake
NETL
NSTL
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