Gynostemma pentaphyllum polysaccharides enhance the immunologic function of mouse peritoneal macrophages
In order to investigate the effects of different concentrations(12.5,25.0,50.0,100.0,200.0 μg/mL)of Gynostemma pen-taphyllum polysaccharides(GPP)on mouse peritoneal macrophages,and to explore the regulatory effect of GPP on immunologic func-tion and its possible mechanisms,the neutral red method,Griess method,CCK8 method,ELISA method,and fluorescence quantita-tive PCR method to detect the phagocytic ability,proliferation,the secretion of cytokines(NO,LDH,TNF-α,IL-1β),and CaM,and GSα gene expression level of mouse peritoneal macrophages.The results showed that GPP activated mouse peritoneal macrophages,and the proliferation ability of mouse peritoneal macrophages increased with the increase of GPP concentration.When the GPP concen-tration was 50.0,100.0,and 200.0 μg/mL,the absorbance showed significant differences compared to the blank control treatment;af-ter GPP stimulation,the phagocytic ability of mouse peritoneal macrophages was stronger than that of the blank control treatment.When the GPP concentration was 200.0 μg/mL,the phagocytic ability of mouse peritoneal macrophages was the strongest;GPP promot-ed the secretion of NO,LDH,TNF-α,and IL-1β in mouse peritoneal macrophages;GPP upregulated CaM and GSα gene expressions.When the GPP concentration was 100.0 and 25.0 μg/mL,CaM and GSα gene expression levels had reached their highest levels respec-tively.
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