Exploring the differences in ileal metabolites of laying hens under two different dietary patterns based on non targeted metabolomics
To investigate the differences in metabolites in the ileum contents of laying hens fed with two different dietary conditions:basal diet(CON)and fermented feed containing 5.0%probiotics(PFF),a combination of liquid chromatography-mass spectrometry and non targeted metabolomics was used to detect metabolites in the ileum of laying hens under two different dietary conditions.Differ-ential metabolite screening and KEGG enrichment analysis were performed to screen for differential metabolites and metabolic path-ways in the ileum of laying hens.The results showed that compared with the CON group,the content of bile acids such as bile acid,taurine goose deoxycholic acid,and goose deoxycholic acid in the PFF group was significantly reduced,while the content of small pep-tides such as isoleucine-leucine-glycine-alanine,phenylalanine-glutamate-valine-glutamate,and leucine-tyrosine-threonine-ly-sine was significantly increased;KEGG pathway enrichment analysis showed that metabolites were mainly related to pathways such as bile secretion and primary bile acid biosynthesis.Primary bile acids played important roles in the digestion and metabolism of host lip-ids and lipophilic substances,antioxidant status,cholesterol metabolism,and host-microbial interactions.Adding probiotic ferment-ed feed to the diet of laying hens could improve intestinal absorption function by altering intestinal metabolites.
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