首页|十种芳香南药精油抑制真菌生长和毒素累积的效果比较

十种芳香南药精油抑制真菌生长和毒素累积的效果比较

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研究香茅[Cymbopogon nardus(L。)Rendle]等10种芳香南药精油对黄曲霉菌(Aspergillus flavus)、黑曲霉菌(Aspergillus niger)生长和黄曲霉毒素B1、赭曲霉毒素A累积的抑制效果,以期将芳香南药精油开发为绿色、高效的杀菌剂。水蒸气蒸馏法提取精油,熏蒸法研究精油对黄曲霉菌和黑曲霉菌的抑制作用,气相色谱-质谱法分析精油成分,超高效液相色谱法检测黄曲霉毒素B1和赭曲霉毒素A的含量。结果表明,10种精油的抑菌和抑制毒素累积作用存在较大差异。肉桂精油对黄曲霉菌、黑曲霉菌生长和毒素累积的抑制作用最强。香茅精油、大叶丁香精油、广藿香精油、降香精油对霉菌生长和毒素累积也有较强的抑制作用。益智精油、高良姜精油、白胡椒精油、莪术精油、沉香精油的抑菌效果不明显。甲基丁香酚、广藿香醇、柠檬醛、肉桂醛、反式橙花叔醇及其氧化物可能是发挥抗菌作用的成分。香茅、大叶丁香、广藿香、肉桂和降香精油具有开发为抑菌剂的潜力。
Efficacy comparison of ten essential oils of aromatic southern medicines on the inhibition of fungal growth and mycotoxin accumulation
The efficacy comparison of ten essential oils on the inhibition of fungal growth and mycotoxin accumulation was carried out to provide reference for the development of green and efficient bacteriostatic agents.The essential oils were extracted by steam distilla-tion method and analyzed by gas chromatography-mass spectrometry(GC-MS).Fumigation was used to test the inhibition on Aspergil-lus flavus,Aspergillus niger of essential oils.Aflatoxin B1(AFB1)and ochratoxin A(OTA)were determined by ultrahigh-performance liquid chromatography-triple quadrupole tandem mass spectrometry(UPLC-MS/MS).The results showed that,significant differences were found in the antifungal and antitoxin accumulation effects of ten essential oils.Cinnamon oil could completely inhibit the growth of A.flavus,A.niger and mycotoxins accumulation,while essential oils of Cymbopogon nardus,Dalbergia odorifera,Pogostemon cablin and Eugenia cayophyllata could inhibit the growth of the two fungi and mycotoxins.However,the antifungal effect of other essential oils was not significant.Methyl eugenol,patchouli alcohol,citral,cinnamaldehyde,trans-nerolidol and its oxides might be the com-ponents that exerted antifungal effects.Fragrance essential oils had the potential to be developed as green preservatives.

aromatic southern medicinesessential oilsantimicrobial activityAspergillus flavusAspergillus nigermycotoxins

梁焕宴、邱淑芳、冼梦婷、周亚奎、赵祥升

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中国医学科学院药用植物研究所海南分所/海南省南药资源保护与开发重点实验室,海口 570311

芳香南药 精油 抑制能力 黄曲霉菌 黑曲霉菌 真菌毒素

海南省重点研发项目国家自然科学基金项目海南省自然科学基金人才项目

ZDYF2020183820741302019RC342

2024

湖北农业科学
湖北省农业科学院 华中农业大学 长江大学 黄冈师范学院

湖北农业科学

CSTPCD
影响因子:0.442
ISSN:0439-8114
年,卷(期):2024.63(7)
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