一株猪流行性腹泻病毒变异株的分离、鉴定与全基因组序列分析
Isolation,identification and whole genome sequence analysis of a porcine epidemic diarrhea virus variant strain
刘曦 1贾相超 1李晨曦 1李自力2
作者信息
- 1. 华中农业大学动物医学院,武汉 430070
- 2. 华中农业大学动物医学院,武汉 430070;湖北省预防兽医学重点实验室,武汉 430070;农业微生物学国家重点实验室,武汉 430070
- 折叠
摘要
江苏省某猪场疑似发生猪流行性腹泻(Porcine epidemic diarrhea,PED),采集病料后,经RT-PCR检测PEDV呈阳性.病毒感染Vero细胞能够引起明显的细胞病变,间接免疫荧光鉴定结果显示病毒感染细胞后能被抗PEDV N蛋白的特异性单克隆抗体识别.通过全基因组序列分析,鉴定该毒株为PEDV G2b亚型变异株,并将其命名为PEDV JS-B株.
Abstract
PED was suspected to have occurred in a pig farm in Jiangsu Province,and after collection of disease material,PEDV was tested positive by RT-PCR.Virus-infected Vero cells were able to cause significant cytopathic changes,and the immunofluorescence results showed that the virus-infected cells could be recognized by a specific monoclonal antibody against PEDV N protein.Through whole genome sequence analysis,this strain was identified as a PEDV G2b subtype variant and designated PEDV JS-B strain.
关键词
猪流行性腹泻病毒/分离鉴定/变异毒株/分段测序/序列分析Key words
porcine epidemic diarrhea virus/isolation and identification/variant strain/segmental sequencing/sequence analysis引用本文复制引用
出版年
2024
NETL
NSTL
万方数据