Isolation,identification and whole genome sequence analysis of a porcine epidemic diarrhea virus variant strain
PED was suspected to have occurred in a pig farm in Jiangsu Province,and after collection of disease material,PEDV was tested positive by RT-PCR.Virus-infected Vero cells were able to cause significant cytopathic changes,and the immunofluorescence results showed that the virus-infected cells could be recognized by a specific monoclonal antibody against PEDV N protein.Through whole genome sequence analysis,this strain was identified as a PEDV G2b subtype variant and designated PEDV JS-B strain.
porcine epidemic diarrhea virusisolation and identificationvariant strainsegmental sequencingsequence analysis
NETL
NSTL
万方数据
