Optimization of extraction of luteolin from millet pepper by response surface methodology
With millet pepper as raw material,ultrasonic-assisted extraction was used to extract luteolin,and the extraction rate of lu-teolin was used as the index.The effects of material to liquid ratio,ethanol concentration,extraction time and extraction temperature on the extraction rate of luteolin were investigated through single factor experiment,and the process was optimized by the response sur-face analysis method.The results showed that the optimum extraction condition of luteolin from millet pepper was extraction tempera-ture of 52.3℃,material to liquid ratio of 1∶50,ethanol concentration of 97%,and extraction time of 20 min.Under this extraction condition,the extraction rate was 1.641%,with a deviation of-0.061%compared with the theoretical value.The antibacterial activity of luteolin was tested by the Oxford cup method.The results showed that luteolin extract had a bacteriostatic effect on Staphylococcus aureus and Bacillus subtilis,but had no bacteriostatic effect on Escherichia coli.
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