Simultaneous determination of organic acids and VC content in fruits and vegetables using high performance liquid chromatography
Using a 1%oxalic acid(containing 10%methanol)solution to extract organic acids(tartaric acid,citric acid,malic acid,succinic acid,fumaric acid)and VC from fruits and vegetables,the chromatographic column was InertSustain C18(250 mm×4.6 mm,5 μm),with 0.1%phosphoric acid aqueous solution as mobile phase A and methanol as mobile phase B,gradient elution,injection volume of 20 μL,column temperature of 30℃,and flow rate of 0.8 mL/min.The content of 5 organic acids and was detected VC at wavelengths of 210 nm and 254 nm,respectively.The results showed that the method had a good linear relationship within the stan-dard curve range,with correlation coefficients(R2)all greater than 0.999 0,detection limits of 0.01～0.90 μg/mL,quantification lim-its of 0.15～5.00 μg/mL,relative standard deviation of 2.40%～3.75%,and recovery rates of 86.4%～115.2%;this method had the ad-vantages of fast analysis speed,high sensitivity,good repeatability,and high efficiency,and could be used for the determination of or-ganic acids and VC content in fruits and vegetables.
high performance liquid chromatographyfruits and vegetablesorganic acidsVCcontent
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