Antifungal effect of canthin-6-one from Ailanthus altissima on Fusarium graminearum
To further study the fungicidal effect and mechanism of canthin-6-one alkaloid from Ailanthus altissima on Fusarium gra-minearum,the inhibitory activity was determined by microdilution and mycelial growth inhibition methods.The antifungal mechanism of canthin-6-one was analyzed from the point of view of cell membrane by detecting the extracellular relative conductivity,content re-lease,malondialdehyde content,hydrogen peroxide content and protective enzyme activity.The results showed that canthin-6-one had a significant inhibitory effect on Fusarium graminearum,with a minimum inhibitory concentration of 8.0 μg/mL and a half-effec-tive concentration of 1.85 μg/mL.The extracellular relative conductivity and the content release amount of the treated mycelia were sig-nificantly increased,while malondialdehyde and hydrogen peroxide contents were also significantly elevated.It was indicated that can-thin-6-one induced oxidative damage to the cell membrane of Fusarium graminearum,resulting in the loss of membrane integrity and increased permeability,thus inhibiting mycelial growth.
NETL
NSTL
万方数据
