臭椿canthin-6-one对禾谷镰刀菌的抑菌作用
Antifungal effect of canthin-6-one from Ailanthus altissima on Fusarium graminearum
赵美荣 1李永春 1李春颖1
作者信息
- 1. 赤峰学院化学与生命科学学院,内蒙古 赤峰 024000
- 折叠
摘要
为进一步研究臭椿(Ailanthus altissima)canthin-6-one生物碱对禾谷镰刀菌(Fusarium graminearum)的抑菌效果及其作用机制,采用微量稀释法、菌丝生长抑制法测定其抑菌活性,并通过测定胞外相对电导率、内容物释放量、丙二醛含量、过氧化氢含量以及保护酶活性,从细胞膜角度分析其抑菌作用机制.结果显示,canthin-6-one对禾谷镰刀菌具有显著的抑菌作用,其最小抑菌浓度(MIC)为8.0 μg/mL,半数有效浓度(EC50)为1.85 μg/mL.处理后的菌体胞外相对电导率和内容物释放量显著增加,同时菌体丙二醛和过氧化氢含量亦有显著升高.这表明canthin-6-one引起了禾谷镰刀菌细胞膜氧化损伤,导致膜完整性丧失及透性增强,从而抑制菌丝生长.
Abstract
To further study the fungicidal effect and mechanism of canthin-6-one alkaloid from Ailanthus altissima on Fusarium gra-minearum,the inhibitory activity was determined by microdilution and mycelial growth inhibition methods.The antifungal mechanism of canthin-6-one was analyzed from the point of view of cell membrane by detecting the extracellular relative conductivity,content re-lease,malondialdehyde content,hydrogen peroxide content and protective enzyme activity.The results showed that canthin-6-one had a significant inhibitory effect on Fusarium graminearum,with a minimum inhibitory concentration of 8.0 μg/mL and a half-effec-tive concentration of 1.85 μg/mL.The extracellular relative conductivity and the content release amount of the treated mycelia were sig-nificantly increased,while malondialdehyde and hydrogen peroxide contents were also significantly elevated.It was indicated that can-thin-6-one induced oxidative damage to the cell membrane of Fusarium graminearum,resulting in the loss of membrane integrity and increased permeability,thus inhibiting mycelial growth.
关键词
臭椿(Ailanthus/altissima)/禾谷镰刀菌(Fusarium/graminearum)/生物碱/抑菌作用/细胞膜Key words
Ailanthus altissima/Fusarium graminearum/alkaloid/antifungal effect/cell membrane引用本文复制引用
出版年
2024
NETL
NSTL
万方数据