Identification of pathogen of sclerotinia on Sophora tonkinensis Radix and screening of laboratory agents
The pathogens from Sophora tonkinensis Radix were isolated by conventional tissue separation method,the pathogenicity was determined by Koch's formula,and the pathogens were identified based on morphological characteristics,mycelium fusion group determination,nuclear fluorescence staining and rDNA-ITS sequence analysis.The inhibitory activities of 5 fungicides against patho-gens were measured by the mycelium growth rate method.The results showed that the pathogen was Rhizoctonia solani,belonging to the fusion group AG-1.98%hymexazol soluble power(SP),75%oxime·pentazolol water dispersible granule(WG),250 g/L pyra-clostrobin emulsifiable concentrate(EC),1 billion/g Trichoderma harziana suspension concentrate(SC),and 50%carbendazim wet-table power(WP)all had good inhibitory effects on the growth of pathogenic fungi mycelia.The inhibitory medium effective concentra-tions(EC50)were 0.061 1,0.575 7,0.074 8,0.284 3,0.740 5 mg/L,respectively.
Sophora tonkinensis Radixsclerotiniaidentification of pathogenscreening of laboratory agentsRhizoctonia solani
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