山豆根菌核病病原菌鉴定及室内药剂筛选
Identification of pathogen of sclerotinia on Sophora tonkinensis Radix and screening of laboratory agents
宋利沙 1蒋妮 1张占江 1韦树根 1丘卓秋 1黄琦 1詹鑫婕 1潘丽梅1
作者信息
- 1. 广西壮族自治区药用植物园/广西道地药材高品质形成与应用重点实验室/广西中药材良种繁育技术创新中心/国家中医药传承创新中心,南宁 530023
- 折叠
摘要
采用常规组织分离法分离山豆根(Sophora tonkinensis Radix)菌核病病原菌,利用柯赫式法则进行致病性测定,基于形态特征、菌丝融合群测定、细胞核荧光染色以及rDNA-ITS序列分析进行病原菌的鉴定,并利用菌丝生长速率法测定5种杀菌剂对病原菌的抑制活性.结果表明,山豆根菌核病的病原菌为立枯丝核菌(Rhizoctonia solani),属于融合群AG-1.98%恶霉灵可溶性粉剂(SP)、75%肟菌·戊唑醇水分散粒剂(WG)、250 g/L吡唑醚菌酯乳油(EC)、10亿/g哈茨木霉菌悬浮剂(SC)、50%多菌灵可湿性粉剂(WP)对病原菌菌丝的生长均有较好的抑制效果,抑制中浓度(EC50)分别为0.061 1、0.575 7、0.074 8、0.284 3、0.740 5 mg/L.
Abstract
The pathogens from Sophora tonkinensis Radix were isolated by conventional tissue separation method,the pathogenicity was determined by Koch's formula,and the pathogens were identified based on morphological characteristics,mycelium fusion group determination,nuclear fluorescence staining and rDNA-ITS sequence analysis.The inhibitory activities of 5 fungicides against patho-gens were measured by the mycelium growth rate method.The results showed that the pathogen was Rhizoctonia solani,belonging to the fusion group AG-1.98%hymexazol soluble power(SP),75%oxime·pentazolol water dispersible granule(WG),250 g/L pyra-clostrobin emulsifiable concentrate(EC),1 billion/g Trichoderma harziana suspension concentrate(SC),and 50%carbendazim wet-table power(WP)all had good inhibitory effects on the growth of pathogenic fungi mycelia.The inhibitory medium effective concentra-tions(EC50)were 0.061 1,0.575 7,0.074 8,0.284 3,0.740 5 mg/L,respectively.
关键词
山豆根(Sophora/tonkinensis/Radix)/菌核病/病原菌鉴定/室内药剂筛选/立枯丝核菌(Rhizoctonia/solani)Key words
Sophora tonkinensis Radix/sclerotinia/identification of pathogen/screening of laboratory agents/Rhizoctonia solani引用本文复制引用
出版年
2024
NETL
NSTL
万方数据