球孢白僵菌利用D-PPA合成D-HPPA的发酵条件优化
Optimization of fermentation conditions for the synthesis of D-HPPA from D-PPA by Beauveria bassiana
彭继兰 1余杰 1刘宗求 1王金华 1高娃 1王永泽1
作者信息
- 1. 湖北工业大学教育部工业发酵省部共建协同创新中心/发酵工程教育部重点实验室/工业微生物湖北省重点实验室,武汉 430068
- 折叠
摘要
为了提高球孢白僵菌(Beauveria bassiana)利用R-(+)-2-苯氧基丙酸(D-PPA)合成R-(+)-2-(4-羟基苯氧基)丙酸(D-HPPA)的能力,在单因素试验的基础上,通过Plackett-Burman设计和Box-Behnken中心组合设计优化球孢白僵菌生物合成D-HPPA的液态发酵条件.结果表明,当D-PPA初始添加浓度为40 g/L时,液态发酵最优条件为葡萄糖38.70 g/L、蚕蛹粉15 g/L、FeCl21.0 g/L、接种量15%(V/V)、pH 6.6及发酵温度28℃时,D-HPPA的产量为(25.10±0.43)g/L,D-HPPA的产率为57.24%±0.97%,比优化前提高了159%.
Abstract
In order to improve the ability of Beauveria bassiana to synthesize R-(+)-2-(4-hydroxyphenoxy)propionic acid(D-HP-PA)from R-(+)-2-phenoxy propionic acid(D-HPPA),on the basis of single factor test,the liquid fermentation conditions of D-HPPA biosynthesis by Beauveria bassiana were optimized by Plackett-Burman design and Box-Behnken center combination design.The results showed that when the initial concentration of D-PPA was 40 g/L,the optimal conditions for liquid fermentation were glu-cose 38.70 g/L,chrysalis powder 15 g/L,FeCl2 1.0 g/L,inoculation amount 15%(V/V),pH 6.6 and fermentation temperature 28℃.The yield of D-HPPA was(25.10±0.43)g/L,and the yield of D-HPPA was 57.24%±0.97%,which was 159%higher than that before optimization.
关键词
球孢白僵菌(Beauveria/bassiana)/液态发酵/条件优化/R-(+)-2-(4-羟基苯氧基)丙酸/Plackett-Burman设计/Box-Behnken中心组合设计Key words
Beauveria bassiana/liquid fermentation/condition optimization/R-(+)-2-(4-hydroxyphenoxy)propionic acid/Plack-ett-Burman design/Box-Behnken center combination design引用本文复制引用
出版年
2024
NETL
NSTL
万方数据