一株鸭疫里氏杆菌的分离、鉴定与生物学特性分析
Isolation,identification and biological characterization of a strain of Riemerella anatipestifer
王博焘 1郭云清 2张腾飞 2张文婷 2卢琴 2胡巧 2罗青平 3翟新国4
作者信息
- 1. 河北工程大学生命科学与食品工程学院,河北 邯郸 056000;农业农村部畜禽细菌病防治制剂创制重点实验室/畜禽病原微生物学湖北省重点实验室/畜牧兽医研究所,湖北省农业科学院,武汉 430064
- 2. 农业农村部畜禽细菌病防治制剂创制重点实验室/畜禽病原微生物学湖北省重点实验室/畜牧兽医研究所,湖北省农业科学院,武汉 430064
- 3. 农业农村部畜禽细菌病防治制剂创制重点实验室/畜禽病原微生物学湖北省重点实验室/畜牧兽医研究所,湖北省农业科学院,武汉 430064;湖北洪山实验室,武汉 430064
- 4. 河北工程大学生命科学与食品工程学院,河北 邯郸 056000
- 折叠
摘要
为确定引起2022年湖北省襄阳市某规模化鸭场的鸭急性死亡病因,对病料进行病原菌分离、对分离株进行生化特性鉴定、耐药性测定、动物回归试验及全基因组测序.结果表明,急性死亡的鸭病原菌仅有鸭疫里氏杆菌(Riemerella anatipestifer)分离鉴定为阳性.药敏结果显示分离菌株对药物敏感性较高,仅对环丙沙星、氧氟沙星耐药;动物回归试验结果显示分离菌株可引起雏鸭急性死亡,剖检可见典型的浆膜炎症状.组织病理学切片显示心脏、肝脏被膜有明显纤维性渗出,心肌细胞有炎性浸润、脾脏实质内可见淋巴细胞数量明显减少、脑组织可见较多的血管淤血扩张.毒力测定显示分离菌株的半数致死量为3.06×106 CFU/mL.
Abstract
In order to determine the cause of acute death of ducks in a large-scale duck farm in Xiangyang City,Hubei Province in 2022,the pathogenic bacteria were isolated,and the biochemical characteristics,drug resistance,animal regression tests and whole genome sequencing of the isolated strain were identified.The results showed that only Riemerella anatipestifer was isolated and identi-fied as positive in acute dead duck pathogens.The drug susceptibility results showed that the isolated strains had high sensitivity to an-tibiotics and were resistant to ciprofloxacin and ofloxacin.The results of animal regression experiments showed that isolated strains could cause acute death of ducklings,typical symptoms of serositis were seen on autopsy.Histopathological sections showed obvious fi-brous exudation of the heart and liver envelope,inflammatory infiltration of cardiomyocytes,a significant decrease in the number of lymphocytes in the spleen parenchyma,and more vascular congestion and expansion in brain tissue.Virulence assays showed that the half lethal dose of isolated strains was 3.06×106 CFU/mL.
关键词
鸭疫里氏杆菌(Riemerella/anatipestifer)/生化特性/回归试验/病理变化/毒力Key words
Riemerella anatipestifer/biochemical properties/regression testing/pathological changes/virulence引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据