Isolation,identification and biological characterization of a strain of Riemerella anatipestifer
In order to determine the cause of acute death of ducks in a large-scale duck farm in Xiangyang City,Hubei Province in 2022,the pathogenic bacteria were isolated,and the biochemical characteristics,drug resistance,animal regression tests and whole genome sequencing of the isolated strain were identified.The results showed that only Riemerella anatipestifer was isolated and identi-fied as positive in acute dead duck pathogens.The drug susceptibility results showed that the isolated strains had high sensitivity to an-tibiotics and were resistant to ciprofloxacin and ofloxacin.The results of animal regression experiments showed that isolated strains could cause acute death of ducklings,typical symptoms of serositis were seen on autopsy.Histopathological sections showed obvious fi-brous exudation of the heart and liver envelope,inflammatory infiltration of cardiomyocytes,a significant decrease in the number of lymphocytes in the spleen parenchyma,and more vascular congestion and expansion in brain tissue.Virulence assays showed that the half lethal dose of isolated strains was 3.06×106 CFU/mL.
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