QuEChERS method combined with high performance liquid chromatography tandem mass spectrometry for detecting residues of 10 carbamate pesticides in beer
To establish a QuEChERS high-performance liquid chromatography tandem mass spectrometry method for simultaneous de-termination of 10 carbamate pesticide residues in beer,beer samples were extracted with acetonitrile,and 1.5 g of sodium chloride was used for salting out.The purification agent was 30 mg PSA+15 mg GCB+885 mg anhydrous magnesium sulfate,mobile phase A was 0.01%formic acid aqueous solution+2 mmol/L ammonium formate solution,mobile phase B was methanol,and the chromatographic column was Shim pack GIST-HP C18-AQ(100 mm×2.1 mm,1.9 μm).The analysis was carried out in multiple reaction monitoring mode(MRM)and the external standard method was used for quantification.The results showed that there was a good linear relation-ship among the 10 types of carbamate pesticides within the concentration range of 5～150 μg/L,and the correlation coefficients(R2)were all greater than 0.999 0,the LOD was 0.10-1.43 μg/L,the LOQ was 0.29-4.76 μg/L,the spiked recovery rate of the sample was 85.9%～118.0%,and the RSD was 1.4%～8.8%(n=6).This method was accurate in quantification,simple in operation,and fast in analysis,and was suitable for rapid screening and quantitative analysis of various carbamate pesticide residues in beer.
beerQuEChERS methodhigh performance liquid chromatography tandem mass spectrometrycarbamate pesticidesresidues
NETL
NSTL
万方数据
