Simultaneous determination of organic selenium and inorganic arsenic in selenium rich rice by LC-ICP-MS method
Using liquid chromatography inductively coupled plasma mass spectrometry(LC-ICP-MS)technology,a method for simul-taneous detection of organic selenium and inorganic arsenic in selenium-rich rice had been established,which was used to detect the content of organic selenium and inorganic arsenic in selenium-rich rice sold in Hubei Province.The pre-treatment method for seleni-um rich rice involved the mixed extraction by using of 15 mg proteinase E and 15 mg proteinase K,the chromatographic conditions were performed using an AgClient ZORBAX SB Aq column,and the mobile phase consistd of a 20 mmol/L citric acid solution,a 5 mmol/L sodium hexane sulfonate solution,and a 1%methanol solution(pH=4.0).Sample in jection was conducted at a flow rate of 1 mL/min,mass spectrometry used HEHe mode,and three types of organic selenium and two types of inorganic arsenic were separated completely within 5 minutes.The three types of organic selenium and two types of inorganic arsenic showed a good linear relationship ranging from 1 to 50 μg/L,with correlation coefficients(R2)greater than 0.999 0;the detection limits of As(Ⅴ),As(Ⅲ),SeCys2,MeSeCys,and SeMet were 0.10,0.12,0.18,0.15,and 0.15 μg/L,respectively,which met the detection requirements.The recovery rate of inorganic arsenic was 96.2%～101.3%,with a relative standard deviation of 2.5%～3.9%;the recovery rate of organic selenium was 72.4%～100.6%,with a relative standard deviation of 2.1%～4.1%.This method was easy to operate,fast,and highly sensitive,and could be used for simultaneous detection of organic selenium and inorganic arsenic in selenium rich rice.
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