Identification of drought resistance of polyploid Oryza sativa L.DHF-CBS gene
In order to explore the drought resistance mechanism of DHF-CBS gene regulation in polyploid Oryza sativa L.under drought stress,two varieties of Oryza sativa L.were selected,namely polyploid plant Zhonghua-11(DHF-CBS gene)and mutant plant UC-32(non expressing DHF-CBS gene),the GUS chemical staining results,tissue moisture content,abscisic acid(ABA)con-tent,thiobarbituric acid reactive substance(TBARS)content,glutathione(GSH)content,etc.of two types of Oryza sativa L.were analyzed.The results showed that on the 7th day of drought stress,some leaves of Zhonghua-11 remained green and firm.On the 11th day of drought stress,the entire plant's leaves turned yellow,and some leaves even withered,after observing the phenotype under drought stress,it was found that Zhonghua-11 had stronger drought resistance than UC-32.Analysis of the water content,ABA con-tent,TBARS content,and GSH content of Oryza sativa L.showed that the drought resistance of Zhonghua-11 was better than that of UC-32,indicating that the DHF-CBS gene enhanced the drought resistance of Oryza sativa L..
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