Separation and identification of pathogenic fungi causing Chinese cabbage black leaf spot disease in Shiyan City
The pathogenic fungi of the black spot of Brassica pekinensis in Shiyan City,Hubei Province were identified and analyzed,and the corresponding preventive measures were proposed.The pathogenic fungi were isolated from diseased leaves by tissue separa-tion and identified by rDNA ITS and Alt-α1 gene sequence alignment.The results showed that a new Alternaria sp.fungus was found,which was presumed to be the pathogenic fungus causing the local cabbage black spot disease in Shiyan City.In addition,it was con-firmed that the joint analysis of ITS sequence and Alt-α1 gene sequence could distinguish three common Alternaria fungi:Alternaria Brassicola,Alternaria japonica and Alternaria brassicae.
Chinese cabbage(Brassica pekinensis)rDNA ITSblack leaf spot diseasepathogenic fungiseparation and identifica-tionShiyan City,Hubei Province
万方数据
维普
