Effects of vacuum treatment and bud period on Zinnia elegans inflorescence dipping method
Based on the observation of the head inflorescence morphology and ovary cytology of Zinnia elegans S5,the applicability of the transformation technology of Zinnia elegans was explored by using different ratios of Agrobacterium suspension,vacuum treatment method and non-tissue culture inflorescence infection method during the development of the head inflorescence.The results showed that the external morphology and cytological observation of S5 inflorescence development found that the carpels of small flower bud 7 and later development stages were in an open state.The bud 3 capitate inflorescence was treated with the Agrobacterium suspension of 1/2 MS+0.03%Silwet L-77+10%sucrose+0.01 mg/L 6-BA(OD600 nm=0.4),the obtaining 0.35%conversion rate.The vacuum in-termittent treatment was beneficial to the improvement of the conversion rate.The better treatment combination was vacuum intermit-tent 2 min+30 s treatment bud 5 to bud 7 capitulum.The conversion rate of small bud 7 to bud 9 could reach 3.23%～5.00%.
Zinnia elegansinflorescence dippinggenetic transformationvacuum treatmentbud period
万方数据
维普
