Effects of dietary peony seed shell on the liver histopathology,antioxidant activity and nonspecific immunity of Pelteobagrus fulvidraco
To investigate the effects of dietary peony seed shell on fish oxidation resistance and nonspecific immunity,yellow catfish were fed with 1%or 5%peony seed shell(commercial feed containing 1%or 5%peony seed shell)for 3 weeks.Meanwhile,fish fed with commercial feed only were used as the blank control group,and fish fed with 1%or 5%peony seed oil were used as positive con-trol group.The results showed that,compared with blank control group,the activity of superoxide dismutase(P<0.001)and the con-tent of complement C3(P<0.05)in serum of fish fed with 1%peony seed shell increased significantly.Liver superoxide dismutase and catalase activities were higher than those of the blank control group(P<0.001),and liver malondialdehyde content decreased signifi-cantly(P<0.001).Liver histomorphology of yellow catfish was normal and intact in both the blank control group and 1%peony seed shell group,and there was no obvious difference between the two groups.Peony seed shell had a dose-dependent positive effect on pro-moting the antioxidant capacity and nonspecific immunity in yellow catfish,all the test indexes in the 5%peony seed shell group showed a remarkably better change.Compared with blank control group,serum superoxide dismutase,liver superoxide dismutase and catalase activities in 5%peony seed shell group increased by 100%(P<0.001),245%(P<0.001)and 176%(P<0.001),respective-ly.Serum lysozyme activity and complement C3 content increased by 36%(P<0.001)and 33%(P<0.01),respectively.In both dose groups,peony seed shell showed similar positive effects on yellow catfish compared to peony seed oil,the suitable addition amount was 1%～5%.
万方数据
维普
