Heterologous overexpression of CsLOX6 and CsHPL2 genes in Arabidopsis thaliana enhances plant salt and drought tolerance
To explore the effects of CsLOX6 gene and CsHPL2 gene on the growth of Arabidopsis thaliana and its resistance to drought and salt stress,wild-type Arabidopsis thaliana(WT),transgenic Arabidopsis thaliana(OE-CsLOX6,OE-CsHPL2),and mutant Ara-bidopsis thaliana(TB-Atlox5)were used as materials,and the drought and salt tolerance of the four Arabidopsis thaliana species were evaluated through phenotype observation,transcriptome,and metabolomics analysis.The results showed that the aboveground fresh weight and root length of OE-CsLOX6 were significantly higher than those of other genotypes,and the underground fresh weight was significantly higher than that of other genotypes;the aboveground fresh weight,underground fresh weight,and root length of OE-CsH-PL2 plants were significantly lower than those of other genotypes.There were significant differences in the metabolite content between OE-CsLOX6,OE-CsHPL2,and WT.OE-CsLOX6 and WT had 64 differential metabolites,of which 6 were upregulated and 58 were downregulated;OE-CsHPL2 and WT had 63 differential metabolites,of which 9 were upregulated and 54 were downregulated.Overex-pression of CsLOX6 gene and CsHPL2 gene could enhance the resistance of Arabidopsis thaliana to salt stress and drought stress.Under salt stress and drought stress,compared with wild-type plants,the malondialdehyde content of OE-CsLOX6 and OE-CsHPL2 was sig-nificantly lower,and the degree of membrane peroxidation was lower,the activities of SOD,POD,and CAT enzymes were significant-ly higher than those of the wild type,which improved the antioxidant capacity of the plants and resulted in significantly better leaf growth compared to the wild type plants.
万方数据
维普
