Screening and control effect of highly effective antagonistic bacteria against cotton verticillium wilt
Using Verticillium dahliae Kleb as indicator bacteria,the biocontrol strains with high activity were screened by confronation culture and metabolite inhibition test.The preventive effect of the tested strain was determined by pot experiment.The results showed that 162 strains of bacteria with biocontrol effect were screened from more than 1 000 strains isolated from cotton rhizosphere soil,and 4 strains with good comprehensive effect were obtained by using the plate face-off method.Strains XJ5-6 and XJ6-96 showed better in-hibitory effects,with the band width exceeding 5.0 mm and the inhibitory rate reaching 67.54%and 66.86%,respectively.The results of pot experiment showed that XJ5-6 and XJ6-96 combined inoculation had a good control effect on verticillium wilt of cotton,and the control effect was 32.2%and 29.9%on 28 and 40 days,respectively.XJ6-96 could improve the germination rate of cotton seed.
NETL
NSTL
万方数据
