Creation of radish cytoplasmic male sterility(Ogura CMS)Chinese cabbage breeding material with heat tolerance,clubroot resistance
In order to obtain Ogura cytoplasmic male sterile Chinese cabbage materials with heat resistance and clubroot resistance,F1 was obtained by crossing heat resistant Chinese cabbage as female parent and clubroot resistant baby cabbage as male parent.After identification,CMSF1 was obtained by crossing with Ogura cytoplasmic male sterile baby cabbage with clubroot resistance.Then,F2 obtained by self-crossing F1 was selected as male parent,and CMSF1 was selected as female parent for backcrossing to obtain BC1.The results of phenotypic identification of clubroot resistance showed that F1,CMSF1 and BC1 were resistant to clubroot.The results of mo-lecular marker identification showed that F1 plants carried clubroot resistance genes,and most of CMSF1 and BC1 plants carried club-root resistance genes.The results of heat tolerance identification showed that the increase of SOD,POD and CAT activity of F1,CMSF1 and BC1 under high temperature stress was greater than that of Mini Yellow 1.The floral organ observation of CMSF1 and BC1 showed that the stamen abortion was without pollen,which was Ogura cytoplasmic male sterile strain.
Chinese cabbageclubroot resistanceheat toleranceOgura CMS
NETL
NSTL
万方数据
