耐热、抗根肿病、萝卜胞质雄性不育(Ogura CMS)大白菜材料的创制
Creation of radish cytoplasmic male sterility(Ogura CMS)Chinese cabbage breeding material with heat tolerance,clubroot resistance
赵雅兰 1胡靖锋 2杨红丽 2董相书 3和江明2
作者信息
- 1. 云南大学农学院,昆明 650504;云南省农业科学院园艺作物研究所,昆明 650205
- 2. 云南省农业科学院园艺作物研究所,昆明 650205;国家蔬菜改良中心云南分中心,昆明 650205
- 3. 云南大学农学院,昆明 650504
- 折叠
摘要
为获得耐热、抗根肿病的Ogura胞质雄性不育(Ogura CMS)大白菜(Brassica rapa L.ssp.Pekinensis)材料,以耐热大白菜为母本,抗根肿病娃娃菜为父本获得F1,经鉴定后和抗根肿病Ogura胞质雄性不育娃娃菜杂交,获得CMSF1,再将F1自交获得的F2经鉴定筛选后作为父本,CMSF1鉴定筛选后作为母本进行回交,得到BC1.抗根肿病表型鉴定结果显示F1、CMSF1、BC1表现为抗病,分子标记鉴定结果显示,F1植株均携带根肿病抗性基因,CMSF1 和BC1 大部分植株携带根肿病抗性基因.耐热鉴定结果表明,F1、CMSF1、BC1在高温胁迫下超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)的活性增幅大于迷您黄1号.CMSF1和BC1花器官观察结果表明雄蕊败育无花粉,是Ogura胞质雄性不育株.
Abstract
In order to obtain Ogura cytoplasmic male sterile Chinese cabbage materials with heat resistance and clubroot resistance,F1 was obtained by crossing heat resistant Chinese cabbage as female parent and clubroot resistant baby cabbage as male parent.After identification,CMSF1 was obtained by crossing with Ogura cytoplasmic male sterile baby cabbage with clubroot resistance.Then,F2 obtained by self-crossing F1 was selected as male parent,and CMSF1 was selected as female parent for backcrossing to obtain BC1.The results of phenotypic identification of clubroot resistance showed that F1,CMSF1 and BC1 were resistant to clubroot.The results of mo-lecular marker identification showed that F1 plants carried clubroot resistance genes,and most of CMSF1 and BC1 plants carried club-root resistance genes.The results of heat tolerance identification showed that the increase of SOD,POD and CAT activity of F1,CMSF1 and BC1 under high temperature stress was greater than that of Mini Yellow 1.The floral organ observation of CMSF1 and BC1 showed that the stamen abortion was without pollen,which was Ogura cytoplasmic male sterile strain.
关键词
大白菜(Brassica/rapa/L.ssp.Pekinensis)/根肿病/耐热/Ogura胞质雄性不育Key words
Chinese cabbage/clubroot resistance/heat tolerance/Ogura CMS引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据