千屈菜不同部位总黄酮含量及体外抗氧化和降血糖活性
Contents of total flavonoids in different parts of Lythrum salicaria L.and their antioxidant and hypoglycemic activities in vitro
张勇 1庄远杯 1聂华 1翟明 1叶德泳 1张声源1
作者信息
- 1. 嘉应学院医学院,广东 梅州 514031
- 折叠
摘要
探究千屈菜(Lythrum salicaria L.)根、叶、花3个部位总黄酮含量及体外抗氧化和降血糖活性差异,采用硝酸铝法测定总黄酮含量;通过铁离子还原能力、DPPH(1,1-二苯基-2-三硝基苯肼)和ABTS(2,2'-联苯基-1-氨基-三甲基六碳氢阳离子自由基)自由基清除能力评价体外抗氧化活性;利用α-葡萄糖苷酶和α-淀粉酶的抑制作用评价体外降血糖活性;采用Pearson法分析总黄酮含量与活性的相关性.结果表明,千屈菜花总黄酮含量最高[(36.80±0.10)mg RT/g],清除DPPH自由基能力[IC50为(0.009 1±0.000 3)mg/mL]、清除ABTS自由基能力[IC50为(0.074 4±0.002 0)mg/mL]、铁离子还原能力[(2 670.78±9.43)μmol VC/g]和对α-葡萄糖苷酶的抑制作用[IC50为(0.567±0.012)mg/mL]最强;千屈菜根对α-淀粉酶的抑制作用[IC50为(0.051±0.003)mg/mL]最强.千屈菜根、叶、花的总黄酮含量与抗氧化活性呈极显著正相关,与降血糖活性呈显著正相关.千屈菜花显示出较强的抗氧化和α-葡萄糖苷酶的抑制作用,根具有较强α-淀粉酶的抑制作用.
Abstract
The contents of total flavonoids in roots,leaves and flowers of Lythrum salicaria L.and their antioxidant and hypoglycemic activities in vitro were investigated.The content of total flavonoids was determined by the aluminum nitrate method.The antioxidant ac-tivity in vitro was evaluated by iron ion reduction ability,DPPH(1,1-diphenyl-2-trinitrophenylhydrazine)and ABTS(2,2′-biphe-nyl-1-amino-trimethylhexahydrocation)free radical scavenging ability.The hypoglycemic activity in vitro was evaluated by the inhibi-tion of α-glucosidase and α-amylase.Pearson method was used to analyze the correlation between total flavonoid content and activity.The results showed that the content of total flavonoids in Lythrum salicaria L.flowers was the highest[(36.80±0.10)mg RT/g],the DPPH free radical scavenging ability[IC50(0.009 1±0.000 3)mg/mL],ABTS free radical scavenging ability[IC50(0.074 4±0.002 0)mg/mL],iron ion reduction ability[(2 670.78±9.43)μmol VC/g]and the inhibition of α-glucosidase[IC50(0.567±0.012)mg/mL]were the strongest.The inhibitory effect of Lythrum salicaria L.roots on α-amylase[IC50(0.051±0.003)mg/mL]was the strongest.The content of total flavonoids in roots,leaves and flowers of Lythrum salicaria L.was highly significantly positively correlated with antioxi-dant activity and significantly positively correlated with hypoglycemic activity.Lythrum salicaria L.flowers showed strong antioxidant and α-glucosidase inhibitory effects,and the root had strong α-amylase inhibitory effects.
关键词
千屈菜(Lythrum/salicaria/L.)/总黄酮/抗氧化活性/降血糖活性Key words
Lythrum salicaria L./total flavonoids/antioxidant activity/hypoglycemic activity引用本文复制引用
出版年
2024
NETL
NSTL
万方数据