Development of new Hongshancaitai line with clubroot resistance
Using clubroot resistance(CR)Brassica rapa ssp.chinensis var.purpurea germplasm GD1 as the resistant donor,and using DGZ1902,an early mature strain of Hongshancaitai,as the background material,backcross breeding was conducted.The penta-prim-er amplification refractory mutation system(PARMS)marker for CR gene CRzi8 carried by CR donor was developed and furtherly ap-plied in the marker assistance selection(MAS),single plant selection and identification of root disease inoculation,finally the new CR Hongshancaitai line was created.The results showed that the PAMRS marker for CRzi8 could accurately detect the CR individuals from the backcross breeding program.Field resistance test and agronomic traits evaluation indicated that the developed CR Hongshan-caitai line CR-DGZ1902 was resistant to Plasmodiophora brassicae pathotype 4,and had both agronomic characteristics of Hongshan-caitai and early maturity characteristics of DGZ1902.
Hongshancaitaimolecular markerclubroot diseaseresistance genenew line
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