鮟鱇鱼肉多肽对小鼠急性毒性试验
Acute toxicity test of peptides from monkfish meat in mice
沈露佳 1沈梦冉 1何佳蔚 1唐晶 1张玺 1杨最素1
作者信息
- 1. 浙江海洋大学食品与药学学院,浙江 舟山 316022
- 折叠
摘要
为探讨鮟鱇鱼(Lophius litulon)肉多肽对小鼠的急性毒性作用,将健康的雄性清洁级ICR小鼠18只,随机分为对照组、LPs低剂量组(2.5 g/kg)和LPs高剂量组(10 g/kg)3个组.每只小鼠按20 mL/kg进行灌胃,连续观察14 d,记录小鼠初始体重、第7天体重及第14天体重,观察小鼠的中毒症状以及死亡情况.试验结束后小鼠眼球取血检测其肝肾功能相关指标,取心脏、肝脏、肾脏、脾脏、肺观察外观及HE染色后组织结构的变化.结果表明,3组小鼠均未出现死亡,肝肾功能相关指标与对照组相比无显著差异(P>0.05).LPs高剂量组和LPs低剂量组小鼠心脏、肝脏、肾脏、脾脏、肺等外观正常,HE染色后组织结构清晰,未见到异常变化,鮟鱇鱼肉多肽对小鼠无急性毒性作用.
Abstract
To investigate the acute toxicity of monkfish(Lophius litulon)polypeptides(LPs)in mice,eighteen healthy male ICR mice were randomly divided into three groups:control group,LPs low-dose group(2.5 g/kg),and LPs high-dose group(10 g/kg).Each mouse was administered with 20 mL/kg of LPs through gavage,and they were observed continuously for 14 days.The initial weight at the start of the experiment,weight on the 7th day,and weight on the 14th day were recorded.Signs of toxicity and mortality were also observed.At the end of the experiment,blood was collected from the mice's eyeballs to test for liver and kidney function-related indi-cators.Heart,liver,kidney,spleen,and lungs were collected for external observation,and the tissues were subjected to HE staining for histological analysis.The results showed that no deaths occurred in any of the three groups of mice.Liver and kidney function-relat-ed indicators showed no significant differences compared to the control group(P>0.05).The heart,liver,kidney,spleen,and lungs of mice in the high-dose and low-dose LPs group appeared normal,and microscopic examination of tissues stained with HE revealed clear structures without abnormal changes.Therefore,Lophius litulon polypeptides had no acute toxicity in mice.
关键词
鮟鱇鱼(Lophius/litulon)/多肽/小鼠/急性毒性Key words
Lophius litulon/polypeptide/mouse/acute toxicity引用本文复制引用
出版年
2024
NETL
NSTL
万方数据