Yantiao Formula protects immune function and intestinal microecology in patients with acute sepsis by regulating lncRNA-Miat
Objective To investigate the protective effects of Yantiao Formula on intestinal microecology and immune function in patients with acute sepsis by regulating long non-coding RNA-myocardial infarction associated transcript(LncRNA-Miat).Methods A total of 108 patients with acute sepsis in our hospital from January 2020 to October 2022 were selected and randomly divided into control group(n=54)and observation group(n=54)according to random number table method.All patients were treated with conventional symptomatic treatment,and those in observation group were additionally given Yantiao Formula.The treatment lasted for 10 days.Six patients in the observation group dropped out due to changes in consciousness or coma.In order to maintain the same number of cases,6 cases were excluded from the control group,and 48 cases in both the observation group and the control group entered the study.The traditional Chinese medicine(TCM)efficacy,TCM syndrome score,LncRNA-Miat,expression levels of phospholipase tensin homology(PTEN),immune function factors(nuclear factor κB[NF-κB],nucleotide binding oligomeric domain like receptor protein 3[NLRP3],interleukin-1β[IL-1β],IL-18),intestinal microecology(Lactobacillus,Enterobacter,Staphylococcus),and 28-day mortality were compared.Results After treatment,the total effective rate of the observation group was significantly higher than that of control group(83.33%[40/48)vs 62.50%[30/48]),and the scores of fever,thirst and cold,sweating,asthma and fatigue were significantly lower than those of the control group(P<0.05).After treatment,expression levels of PTEN,LncRNA-Miat,NF-κB,NLRP3,IL-1β,and IL-18,and relative abundances of Staphylococcus and Enterobacter were significantly lower in the observation group than those of control group,and the relative abundance of Lactobacillus was significantly higher(P<0.05).After treatment,the Acute Physiology and Chronic Health EvaluationⅡ(APACHEⅡ)and the Sequential Organ Failure Assessment(SOFA)scores in observation group were significantly lower than those of control group(P<0.05).There was no significant difference in the 28-day mortality between the two groups(P>0.05).Conclusion The combination of Yantiao Formula in the treatment of acute sepsis can effectively regulate the expression of LncRNA-Miat,improve the balance of intestinal microorganisms,enhance immunity,alleviate clinical symptoms,and promote the acceptable outcome.
Yantiao Formulalong non-coding RNA-myocardial infarction associated transcript(LncRNA-Miat)acute sepsisintestinal microecologyimmune function
万方数据
维普
