Effects of toosendanin on the proliferation,migration and invasion of thyroid cancer cells by regulating the Notch signaling pathway
Objective To investigate the effects of toosendanin on the proliferation,migration and invasion of thyroid cancer cells by regulating the Notch signaling pathway.Methods Human thyroid cancer cell line 8505C was cultured in vitro,and the effect of different doses of toosendanin on the survival rate of 8505C cells was detected by CCK-8 assay.8505C cells were grouped into control group,toosendanin-L,M,H groups,and toosendanin+DAPT(Notch signaling pathway inhibitor)group.EDU assay was applied to detect the proliferation of 8505C cells.Transwell assay was conducted to detect the migration and invasion abilities of 8505C cells.The reverse transcription-polymerase chain reaction(RT-PCR)was used to detect the mRNA levels of Notch1,HES1,HEY1,Ki67 and matrix metalloproteinase 2(MMP-2)in different 8505C cell groups.Western blot was applied to detect the protein levels of Notch1,HES1,HEY1,Ki67 and MMP-2 in different 8505C cell groups.Results Compared with the control group,the EDU positive cell rate,counts of migration and invasion cells,and the mRNA and protein levels of Ki67 and MMP-2 in 8505C cells in the toosendanin-L,M,and H groups were obviously reduced,while the mRNA and protein levels of Notch1,HES1 and HEY1 were significantly elevated in a dose-dependent manner(P<0.05).Compared with the toosendanin-H group,the EDU positive cell rate,counts of migration and invasion cells,and the mRNA and protein levels of Ki67 and MMP-2 in 8505C cells in the toosendanin+DAPT group increased significantly,whereas the mRNA and protein levels of Notch1,HES1 and HEY1 decreased obviously(P<0.05).Conclusion Toosendanin may inhibit the proliferation,migration and invasion of thyroid cancer 8505C cells by activating the Notch signaling pathway.
toosendaninNotch signaling pathwaythyroid cancer cellsproliferationmigrationinvasion
万方数据
维普
