Effect of TP53INP1-mediated TGF-β1 on the proliferation and migration of bladder cancer cells
Objective To analyze the effect of tumor protein 53 induced nuclear protein 1(TP53INP1)-mediated transformation growth factor-β1(TGF-β1)on the proliferation and migration of bladder cancer cells.Methods The expression levels of TP53INP1 and TGF-β1 were detected in 40 bladder cancer tissues and paracancerous tissues in patients undergoing bladder cancer resection in our hospital from March 2019 to March 2022.Bladder cancer cells were cultured and passaged in vitro.They were divided into TP53inp1-NC group,TP53INP1 group,TGF-β1-NC group,TGF-β1 group,TP53INP1 group +anti-TGF-β1-NC group,and TP53INP1 group +anti-TGF-β1 group based on the specific cell transfection.Cell proliferation,apoptosis and migration were analyzed.Results The expressions of TP53INP1 and TGF-β1 in paracancerous tissues were significantly lower than those of breast cancer carcinoma tissues(P<0.05).Compared with those of TP53inp1-NC group,the expression levels of TP53INP1,B-lymphocytoma-2-associated X protein(Bax),aspartate-specific cysteine protease 3(Caspase-3),and epithelial cadherin(E-cadherin)and apoptotic rate in TP53INP1 group were significantly higher,while the proliferative rate,number of migratory cells and the expression levels of B lymphoblastoma 2 gene(Bcl-2),matrix metalloproteinase-2(MMP-2)and matrix metalloproteinase-9(MMP-9)were significantly lower(P<0.05).Compared with those of TGF-β1-NC group,the expression levels of TGF-β1,Bax,Caspase-3 and E-cadherin and apoptotic rate were significantly higher in TGF-β1 group,while the proliferative rate,number of migratory cells,and expression levels of Bcl-2,MMP-2,and MMP-9 were significantly lower(P<0.05).Dual-luciferase reporter assay showed that compared with that of TGF-β1-NC group,the luciferase activity of WT-TP53INP1 in TGF-β1 group was significantly lower(P<0.05),and that of MUT-TP53INP1 was not significantly changed(P>0.05).Compared with those of TP53INP1 group +anti-TGF-β1-NC group,TP53INP1 group +anti-TGF-β1 group had lower expression levels of TGF-β1,Bax,Caspase-3,and E-cadherin and apoptotic rate,but higher proliferative rate,number of migratory cells,and expression levels of Bcl-2,MMP-2 and MMP-9(P<0.05).Conclusion TP53INP1 may participate in the proliferation,apoptosis and migration of bladder cancer cells by targeting TGF-β1,providing a new direction for the diagnosis and treatment of bladder cancer.
tumor protein 53 induced nucleoprotein 1transforming growth factor-β1bladder cancerproliferatemigration
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