Correlation analysis of serum sKlotho and OPN levels with glucose metabolism disorder and microvascular complications in children with type 1 diabetes mellitus
Objective To investigate the correlation of serum levels of secretory Klotho(sKlotho),and osteopontin(OPN)with glucose metabolism disorder and microvascular complications in children with type 1 diabetes mellitus(TIDM).Methods A total of 116 children with T1DM diagnosed and treated in our hospital from January 2021 to January 2023 were selected as the study subjects(TIDM group).According to the presence or absence of microvascular complications,they were divided into complication group(n=36)and non-complication group(n= 80).Another 60 children with indirect inguinal hernia managed by an elective surgery in our hospital during the same period were selected as the control group.The serum levels of sKlotho and OPN in each group were detected by enzyme linked immunosorbent assay(ELISA).Pearson correlation was performed to analyze the correlation of serum sKlotho and OPN with glucose metabolism indicators in children with T1DM,including fasting plasma glucose(FPG),fasting insulin(FINS),homeostatic model assessment for insulin resistance(HOMA-IR),and glycosylated hemoglobin(HbA1c).Multivariate logistic regression analysis was used to analyze the risk factors for microvascular complications in children with TIDM.The predictive value of serum sKlotho and OPN in the occurrence of microvascular complications in children with TIDM was analyzed by plotting the receiver operating characteristic(ROC)curve.Results The serum sKlotho level in T1DM group was significantly lower than that of control group(t = 42.141,P=0.000).The serum OPN level in T1DM group was significantly higher than that of control group(t=20.629,P=0.000).The serum sKlotho level in the complication group was significantly lower than that in the non-complication group,while the levels of OPN was significantly higher than those of the non-complication group(all P<0.05).In children with T1DM,serum sKlotho was significantly negatively correlated with FPG,FINS,HOMA-IR,and HbA1c,while serum OPN was significantly positively correlated with FPG,FINS,HOMA-IR,and HbA1c(all P<0.05).SKlotho was an independent protective factor for microvascular complications in children with T1DM,while OPN was an independent risk factor for it.The area under the curve(AUC)of the combined detection of serum sKlotho and OPN in diagnosing children with T1DM complicated with microvascular complications was 0.850,which was larger than that of the single detection(AUC=0.778,Z= 5.183;AUC= 0.752,Z= 4.349,respectively;both P<0.001).Conclusion The decrease of sKlotho and the increase of OPN are correlated with the disorder of glucose metabolism in children with T1DM.The combined detection of serum sKlotho and OPN contribute to predict the occurrence of microvascular complications.
万方数据
维普
