MiRNA regulates macrophage polarization in mice with ulcerative colitis by mediating claudin-2
Objective To explore the protective effect of microRNA(miRNA)on mice with ulcerative colitis(UC)and its regulatory effect on macrophage polarization by mediating claudin-2.Methods Female BALB/c mice were randomly divided into control,model,upregulation group and downregulation group based on the body weight,with 15 mice in each group.Colon tissues were subjected to hematoxylin and eosin(H&E)staining.Scores of UC,inflammatory factors,immune cells,macrophages,and claudin-2 expression were analyzed.Results Compared with that of the control group,the expression of miRNA in the model group,upregulation group and downregulation group was significantly higher(P<0.05).Compared with that of the model group,the expression of miRNA in the upregulation group significantly decreased and that in the downregulation group significantly increased(P<0.05).Compared with that of the upregulation group,the expression of miRNA in the downregulation group was significantly higher(P<0.05).Compared with that of the model group,the expression of miRNA on day 3,5 and 7 in UC mice of upregulation group was significantly lower,which was significantly higher in downregulation group(P<0.05).Compared with that of the upregulation group,the expression of miRNA in the downregulation group was significantly higher(P<0.05).Compared with those of the control group,the glycoprotein(CD4+)and leukocyte differentiation antigen(CD8+)of T lymphocytes in the model group,upregulation group,and downregulation significantly increased(P<0.05).Compared with those of the model group,CD4+ and CD8+ levels in the upregulation group significantly decreased,but significantly increased in the downregulation group(P<0.05).Compared with those of the upregulation group,CD4+ and CD8+ levels were significantly higher in the downregulation group(P<0.05).Compared with those of the control group,tumor necrosis factor(TNF-α),interleukin-6(IL-6)and interleukin-8(IL-8)levels in the model group,upregulation group,and downregulation group significantly increased(P<0.05).Compared with those of the model group,TNF-α,IL-6 and IL-8 levels in the upregulation group were significantly lower(P<0.05).Compared with those of the upregulation group,TNF-α,IL-6 and IL-8 levels in the downregulation group were significantly higher(P<0.05).Compared with those of the control group,the level of inducible nitric oxide synthase(iNOS)significantly increased and the macrophage mannose receptor(CD206)significantly decreased in the model group,upregulation group and downregulation group(P<0.05).Compared with those of the model group,the iNOS level in the upregulation group decreased and the CD206 level increased,while the iNOS level in the downregulation group increased and the CD206 level decreased significantly(P<0.05).Compared with those of the upregulation group,the iNOS level in the downregulation group increased and CD206 level decreased significantly(P<0.05).Compared with that of the control group,protein expression of claudin-2 in the model group,upregulation group and downregulation group significantly increased(P<0.05).Compared with that of the model group,protein expression of claudin-2 in the upregulation group decreased,but increased significantly in the down-regulation group(P<0.05).Conclusion Upregulation of ulcerative colitis mice with miRNA is able to regulate macrophage polarization and thereby ameliorates ulcerative colitis.
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