Objective To investigate the intervention effect and mechanism of ginkgo biloba extract in osteoporosis rats based on toll-like receptor 4(TLR4)/nuclear factor-kappaB(NF-κB)pathway.Methods Forty healthy female Sprague-Dawley(SD)rats in the specific pathogen free(SPF)level aged 4-6 weeks were selected and randomly divided into control group(group A),osteoporosis group(group B),positive drug diethylstilbestrol group(group C)and ginkgo biloba extract(group D).After a week of adaptive feeding,modeling was conducted at the second week,and drug intervention was given starting from 3 months of feeding.Group C rats were given 0.05mg/kg of diethylstilbestrol daily,and group D rats were given ginkgo biloba extract at a dose of 200mg/kg per day,all administered by gavage.Group C and D received medication intervention for 6 weeks,while group A and group B received 0.9%sodium chloride solution 1mL/100g orally every day.After 6 weeks of administration,rats were anesthetized and femoral tissues were removed.Alkaline phosphatase(ALP),osteoprotectin(OPG),25-hydroxyvitamin D3(25OHD),tartrate resistant hydrochloric acid phosphatase(TRACP),calcium ions(Ca2+),Magnesium ion(Mg2+),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),catalase(CAT)levels,TLR4 and NF-κB protein expressions were detected.Results Compared to Group A,ALP,TRACP,malondialdehyde(MDA),TLR4,and NF-κB levels increased significantly in groups B,C and D,while the levels of OPG,25OHD,Ca2+,Mg2+,SOD,GSH-Px and CAT decreased obviously(P<0.05).Compared with Group B,levels of ALP,TRACP,MDA,TLR4,and NF-κB in group C and D were significantly reduced,while the levels of OPG,25OHD,Ca2+,Mg2+,SOD,GSH-Px and CAT were obviously elevated(P<0.05).Compared with Group C,the levels of ALP,TRACP,MDA,TLR4,and NF-κB decreased significantly in Group D,whereas the levels of OPG,25OHD,Ca2+,Mg2+,SOD,GSH-Px and CAT increased remarkably(P<0.05).Conclusion Ginkgo biloba extract may improve bone metabolism and oxidative stress response in osteoporosis rats by inhibiting the activation of the TLR4/NF-κB pathway,affect the absorption of osteoclasts and regulate the levels of Ca2+ and Mg2+ in the body,which is of great significance for the treatment of clinical osteoporosis in the future.
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