Objective To investigate the expression levels of glial cell line derived neurotrophic factor receptor alpha 1(GFRA1)and fibrillin-1(FBN1)in osteosarcomas and their clinical significance.Methods A total of 66 osteosarcoma patients hospitalized for surgery from September 2017 to September 2019 in our hospital were collected for fine resection of osteosarcoma tissue samples and paracancerous tissue samples.Clinical data like tumor staging,tumor diameter and tumor differentiation were collected.The positive expressions of GFRA1 and FBN1 were detected by immunohistochemistry.The correlation of protein expressions of GFRA1 and FBN1 in osteosarcomas with the prognosis was analyzed by the Kaplan-Meier method.Multivariate Logistic regression was applied to analyze the prognostic factors for osteosarcoma patients.Results The rate of positively expressed GFRA1 and FBN1 in osteosarcoma tissue were significantly higher than those of paracancerous tissues(P<0.05).The protein expressions of GFRA1 and FBN1 were significantly correlated with clinical staging,differentiation,lung metastasis and soft tissue infiltration of osteosarcoma patients(P<0.05),but not correlated with sex,age,tumor diameter and tumor location(P>0.05).The 3-year survival of patients with positive expressions of GFRA1 and FBN1 in osteosarcoma tissues was significantly lower than that of patients with negative expression of FBN1(P<0.05).Positive expressions of GFRA1 and FBN1,tumor metastasis and soft tissue infiltration were independent risk factors for the prognosis of osteosarcoma patients(P<0.05).Conclusion The expression levels of GFRA1 and FBN1 are correlated with the clinicopathological characteristics and prognosis of osteosarcoma patients,which can be used as prognostic factors for osteosarcoma patients.
关键词
骨肉瘤/GFRA1/FBN1/病理特征
Key words
osteosarcoma/glial cell line derived neurotrophic factor receptor alpha 1(GFRA1)/fibrillin-1(FBN1)/pathological characteristics
维普
万方数据