Effect of miR-429 on inflammatory response and oxidative stress in lung tissue of burned mice
Objective To investigate the effect and possible mechanism of miR-429 on inflammatory response and oxidative stress in lung tissue of burned mice.Methods A total of 24 C57BL/6 mice were randomly divided into Control group,Model group,Negative control group and miR-429 inhibitor group,with 6 mice in each group.Except for Control group,the other three groups of mice were stimulated by hot water to establish a burned model.Before modeling,mice in the miR-429 inhibitor group and Negative control group were injected with the same amount of miR-429 antagomir or miR-NC through tail vein,respectively.Meanwhile,mice in Control group and Model group were given an equal volume of 0.9％ sodium chloride solution.The expression level of miR-429 in lung tissue was detected by quantitative real-time polymerase chain reaction(qRT-PCR).The levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6),malondialdehyde(MDA),superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GSH)in lung tissues were detected by enzyme-linked immunosorbent assay(ELISA).Pathological changes of lung tissue were assessed by the hematoxylin and eosin(H&E)staining.Cell apoptosis was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling(TUNEL)staining.In addition,the related-protein expression associated to nuclear factor erythroid 2-related factor 2(Nrf2)/heme oxygenase-1(HO-1)pathway was measured by western blotting.Results Compared with Control group,the alveolar wall of the Model group widened,with significant tissue edema and inflammatory cell infiltration.The number of apoptotic cells in lung tissue significantly increased,miR-29 expression and the levels of TNF-α,IL-1β,IL-6 and MDA were significantly up-regulated(P<0.05).However,the levels of SOD,CAT and GSH and the protein expressions of Nrf2 and HO-1 decreased significantly(P<0.05).Compared with Negative control group,the pathological lesion of lung tissue in miR-429 inhibitor group was significantly mitigated.The number of apoptotic cells in lung tissue was significantly reduced,miR-429 expression,and the levels of TNF-α,IL-1β,IL-6 and MDA were significantly down-regulated(P<0.05).However,the levels of SOD,CAT and GSH,and the protein expressions of Nrf2 and HO-1 increased significantly(P<0.05).Conclusion Inhibition of miR-429 can repress the inflammatory response and alleviate oxidative stress damage in lung tissue of burned mice,the mechanism of which may be related to the Nrf2/HO-1 signaling pathway.
万方数据
维普
