Impact of astragaloside IV on nerve root injury in rats with lumbar disc herniation by regulating the IL-10/β-EP signaling pathway
Objective To investigate the impact of astragaloside IV on nerve root injury in rats with lumbar disc herniation(LDH)by regulating the interleukin-10(IL-10)/β-endorphin(β-EP)signaling pathway.Methods Ten rats were randomly selected and included in the sham group.The remaining rats were transplanted with the nucleus pulposus to construct a LDH rat model,followed by the grouping into LDH group,astragaloside IV group(20mg/kg astragaloside IV),AS10 group(0.5mg/kg IL-10/β-EP signaling pathway inhibitor AS10),and astragaloside IV+AS10 group(20mg/kg astragaloside IV+0.5mg/kg AS10),with 10 rats in each group.Rats in sham group and LDH group were given an equal amount of 0.9%NaCl solution.Mechanical sensitivity and thermal sensitivity tests were applied to detect the mechanical withdrawal threshold(PWT)and thermal withdrawal threshold(TWL),respectively.The enzyme-linked immunosorbent assay(ELISA)was applied to detect serum levels of interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α).Immunofluorescence staining was applied to determine the activities of microglia and astrocyte.The terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling(TUNEL)staining was applied to detect cell apoptosis.Western blot was applied to detect the protein expressions of IL-10 and β-EP.Results Compared with those of the sham group,rats in LDH group presented significantly lower PWT,TWL,and protein levels of IL-10,and β-EP in the LDH group(P<0.05),but significantly higher apoptosis rate,serum levels of TNF-α and IL-1β,and the numbers of ion calcium junction protein molecule 1(Iba1)and glial fibrillary acidic protein(GFAP)-positive cells(P<0.05).Compared with those of the LDH group,rats in the astragaloside IV group presented significantly higher PWT,TWL,and protein levels of IL-10 and β-EP(P<0.05),but significantly lower apoptosis rate,serum levels of TNF-α and IL-1β,and the numbers of Iba1 and GFAP-positive cells(P<0.05),while the trends in the AS10 group were opposite(P<0.05).AS10 group reversed the improvement effect of astragaloside IV on nerve root injury in LDH rats.Conclusion Astragaloside IV may alleviate inflammation in rats by activating the IL-10/β-EP signaling pathway,thereby alleviating nerve root injury in LDH rats.
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维普
