Impacts of atractylenolide-I on the proliferation,migration and invasion of endometrial carcinoma cells by regulating the IL-6/STAT3 signaling pathway
Objective To investigate the impacts of atractylenolide-I(AT-I)on the proliferation,migration and invasion of endometrial cancer cells by regulating the interleukin-6(IL-6)/signal transducer and activator of transcription 3(STAT3)signaling pathway.Methods The endometrial cancer cell line RL95-2 was induced with AT-I at varying concentrations(0,25,50,75,100 and 150μmol/L),followed by the detection of cell survival rate by MTT assay.Then,RL95-2 cells were induced with blank control,50μmol/L AT-I,10ng/mL IL-6 and 50μmol/L AT-I + 10ng/mL IL-6.Cell proliferation was detected by MTT assay and EdU staining.The relative expressions of IL-6 and STAT3 in cells were detected by quantitative reverse transcriptase PCR(qRT-PCR).Transwell assay was applied to detect cell migration and invasion.The apoptotic rate was detected by flow cytometry Western blot was applied to detect the protein levels of matrix metalloproteinase(MMP)-2,Ki-67,IL-6,p-STAT3 and STAT3.Results Compared with cells induced with blank control,the survival rate of RL95-2 cells treated with 25μmol/L,50μmol/L,75μmol/L,100μmol/L,and 150μmol/L AT-I dose-dependently decreased(P<0.05).Compared with those of the control group,RL95-2 cells induced with 50μmol/L AT-I presented significantly lower cell viability,proliferative rate,invasive and migratory cell numbers,mRNA levels of IL-6 and STAT3 and protein levels of MMP-2,Ki-67,IL-6 and p-STAT3/STAT3,but significantly higher apoptotic rate(all P<0.05).Compared with those of the control group,RL95-2 cells induced with 10ng/mL IL-6 presented significantly higher cell viability,proliferative rate,invasive and migratory cell numbers,mRNA levels of IL-6 and STAT3 and protein levels of MMP-2,Ki-67,IL-6 and p-STAT3/STAT3,but significantly lower apoptotic rate(all P<0.05).Compared with those induced with 50μmol/L AT-I,RL95-2 cells induced with induced with 50μmol/L AT-I + 10ng/mL IL-6 presented significantly higher cell viability,proliferative rate,invasive and migratory cell numbers,mRNA levels of IL-6 and STAT3 and protein levels of MMP-2,Ki-67,IL-6 and p-STAT3/STAT3,but significantly lower apoptotic rate(all P<0.05).Compared with those induced with 10ng/mL IL-6,RL95-2 cells induced with induced with 50μmol/L AT-I + 10ng/mL IL-6 presented significantly lower cell viability,proliferative rate,invasive and migratory cell numbers,mRNA levels of IL-6 and STAT3 and protein levels of MMP-2,Ki-67,IL-6 and p-STAT3/STAT3,but significantly higher apoptotic rate(all P<0.05).Conclusion AT-I may inhibit the proliferation,migration and invasion of the endometrial cancer cell line RL95-2 by inhibiting the IL-6/STAT3 signaling pathway.
atractylenolide-I(AT-I)interleukin-6(IL-6)/signal transducer and activator of transcription 3(STAT3)signal pathwayendometrial carcinomaproliferationmigrationinvasion
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