Analysis of differences in pulmonary microbiota caused by the pulmonary infections of different pathogens
Objective To analyze the differences in pulmonary microbiota in children with pulmonary infections of different pathogens,and to explore the correlation between pulmonary microbiota and different pathogen infections.Methods Bronchoalveolar alveolar lavage fluid(BALF)from children undergoing bronchoscopy for the diagnosis and treatment of pulmonary infections from June 2020 to May 2022 was collected for microbial community detection using the metagenomic next-generation sequencing(mNGS).According to the clinically confirmed pathogen,they were divided into the Mycoplasma pneumoniae pulmonary infection group(MPP group),Streptococcus pneumoniae pulmonary infection group(SPP group)and fungal pulmonary infection group(FP group).NGS detection results of three groups were analyzed.The similarities and differences in pulmonary microbiota at the phylum,genus,and diversity levels were compared.Results There were 25,13 and 16 cases in the MPP group,SPP group and FP group,respectively.No significant differences in age and gender were detected between groups(P>0.05).The dominant microbial communities at the phylum level of the three groups mainly included four types of the Firmicutes,Bacteroides,Proteobacteria and Actinobacteria.The proportion of Bacteroides in MPP group was significantly higher than that of SPP group and FP group(P<0.001).The proportion of Firmicutes in SPP group was significantly higher than that of MPP group and FP group(P<0.001).The proportions of Proteobacteria and Actinobacteria in FP group were significantly higher than those of MPP group and SPP group(P<0.001).The dominant genera of MPP group and SPP group were the Prevotella,Streptococcus and Veillonella,while the dominant genera of FP group were the Streptococcus,Rochella and Streptococcus granulosus.The α diversity of SPP group was significantly higher than that the FP group(P<0.05).The β diversity of SPP group at the genus(P=0.07)and species level(P=0.14)was different from that of MPP and FP groups,with a certain critical significance.Conclusion There are significant differences in the pulmonary microbial flora of children with pulmonary infections of different pathogens.Bacteroides may be conducive to the growth of Mycoplasma pneumoniae,Streptococcus may inhibit the growth of other bacteria,and Proteobacteria and Actinobacteria may be conducive to the growth of fungi.The role of pulmonary microbial needs to be required further studies for treating pulmonary infectious diseases.
万方数据
维普
