Impact of galangin on the malignant biological behaviors of lung cancer cells by regulating the MCP-1/CCR2 signaling pathway
Objective To study the impacts of galangin on the malignant biological behaviors of lung cancer cells by regulating monocyte chemoattractant protein-1(MCP-1)/CC chemokine receptor-2(CCR2)signaling pathway.Methods The human lung cancer cell line A549 was induced with blank control,low-dose galangin(5μmol/L),high-dose galangin(100μmol/L),10μmol/L gefitinib,or high-dose galangin + 75ng/mL MCP-1.Cell viability,apoptosis,migration and invasion were detected by CCK-8 assay,flow cytometry,wound healing assay and Transwell assay,respectively.Protein expressions of MCP-1,CCR2,Caspase-3,Ki67 and matrix metalloproteinase-2(MMP-2)were detected by Western blot.Result Compared with those induced with blank control,A549 cells treated with low-dose galangin,high-dose galangin or gefitinib presented significantly lower cell survival rate,wound healing rate,invasive cell number and protein expressions of MCP-1,CCR2,Ki67 and MMP-2,but higher apoptotic rate and protein expression of Caspase-3(P<0.05).Compared with those induced with high-dose galangin,A549 cells treated with high-dose galangin + 75ng/mL MCP-1 presented significantly higher cell survival rate,wound healing rate,invasive cell number and protein expressions of MCP-1,CCR2,Ki67 and MMP-2,but lower apoptotic rate and protein expression of Caspase-3(P<0.05).Conclusion Galangin may inhibit the malignant biological behaviors of lung cancer A549 cells and promote its apoptosis by inhibiting the MCP-1/CCR2 signaling pathway.
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