Effect of silencing miR-572 via ferroptosis on apoptosis and sorafenib resistance of renal cell carcinoma cells
Objective To explore the role of silencing microRNA-572(miR-572)in renal cell carcinoma cell apoptosis and sorafenib resistance through regulating ferroptosis.Methods The human renal cancer cell line 786-O was induced with blank control,or transfected with miR-572 siRNA,siRNA-NC,miR-572 overexpression plasmid and negative control plasmid using Lipofectamine 2000.Then,three sorafenib-resistant renal cell carcinoma cell lines were created.The expression of miR-572,apoptosis,sorafenib resistance and protein expressions of key proteins in the p53-SAT1-ALOX15 signaling pathway were observed.Results Compared with those transfected with miR-572 overexpression plasmid,miR-572 was significantly downregulated in 786-O cells transfected with miR-572 siRNA(P<0.05).Compared with those transfected with miR-572 overexpression plasmid,786-O cells transfected with miR-572 siRNA presented significantly higher apoptotic rate at 24h and 48h,lower half maximal inhibitory concentration(IC50)value and higher protein expressions of p53,SAT1 and ALOX15(P<0.05).Conclusion Silence of miR-572 promotes ferroptosis and apoptosis of renal cell carcinoma cells and inhibit sorafenib-resistance by activating the p53-SAT1-ALOX15 signaling pathway.
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