Total saponins extracted from Pericarpium trichosanthis reduce airway inflammation in rats with chronic obstructive pulmonary disease by regulating the PI3K/Akt/SIRT1 signaling pathway
Objective To investigate the effect of total saponins extracted from Pericarpium trichosanthis on airway inflammation in rats with chronic obstructive pulmonary disease(COPD)by regulating the phosphatidylinositol 3-kinase(PI3K)/protein kinase B(Akt)/Sirtuin 1(SIRT1)signaling pathway.Methods Rats were randomly divided into control group(blank control),model group(COPD modeling),total saponins group(COPD modeling + 300mg/kg total saponins),LY294002 group(COPD modeling + 0.3mg/kg PI3K inhibitor LY294002),and total saponins + LY294002 group(COPD modeling + 300mg/kg total saponins + 0.3mg/kg LY294002),with 12 rats in each group.Except for the control group,rats in other groups were constructed as COPD models and given drug intervention.After 24 hours of drug intervention,lung function of rats in each group was detected.Giemsa staining was used for the classification and counting of white blood cells in alveolar lavage fluid(BALF).The hematoxylin and eosin(H&E)staining was performed to observe the pathological changes of the lung tissue.Commercial kits were used to detect the levels of interleukin 18(IL-18)and IL-17 in the BALF and serum of rats in each group.Western blotting was used to detect the expressions of proteins in the PI3K/Akt/SIRT1 signaling pathway in the lung tissues of rats in each group.Results Compared with those of control group,rats in the model group presented obvious pathological damages in lung tissues,significantly higher Ri,WBC and IL-18 and IL-17 levels in BALF and serum,and lower MV,PEF and protein expressions of p-PI3K/PI3K,p-Akt/Akt and SIRT1 in lung tissues(P<0.05).Compared with those of model group and total saponins + LY294002 group,rats in the total saponins group presented alleviated pathological damages in lung tissues,significantly lower Ri,WBC and IL-18 and IL-17 levels in BALF and serum(P<0.05),and higher MV,PEF and protein expressions of p-PI3K/PI3K,p-Akt/Akt and SIRT1 in lung tissues(P<0.05).Rats in the LY294002 group presented aggravated pathological damages in lung tissues,significantly higher Ri,WBC and IL-18 and IL-17 levels in BALF and serum,and lower MV,PEF and protein expressions of p-PI3K/PI3K,p-Akt/Akt and SIRT1 in lung tissues(P<0.05).Conclusion The total saponins extracted from Pericarpium trichosanthis can reduce airway inflammation,improve lung tissue damage and repair lung function in COPD rats by activating the PI3K/Akt/SIRT1 signaling pathway.
total saponins extracted from Pericarpium trichosanthisphosphatidylinositol 3-kinase(PI3K)/protein kinase B(Akt)/Sirtuin 1(SIRT1)chronic obstructive pulmonary diseaseairway inflammation
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