Effect of matrine on the proliferation and apoptosis of high-glucose-induced trophoblast cells by regulating the Notch signaling pathway
Objective To investigate the effect of matrine on the proliferation and apoptosis of high-glucose-induced trophoblast cells by regulating the Notch signaling pathway.Methods Human chorionic trophoblast cells HTR-8/SVneo were cultured to the logarithmic phase.They were induced with high glucose,and then treated with different concentrations of matrine.Cell viability was detected by CCK-8 assay.HTR-8/SVneo cells were induced with 5mmol/L glucose,25mmol/L glucose,1.00mmol/L matrine,1.50mmol/L matrine and 1.50mmol/L matrine+500μg/L Notch signaling pathway activator Jagged 1.Cell proliferation and apoptosis were detected by CCK-8 assay and flow cytometry,respectively.Malondialdehyde(MDA)and superoxide dismutase(SOD)levels were detected by commercial kits.Protein expressions of Notch 1,proliferating cell nuclear antigen(PCNA),BAX,B cell lymphoma-2(Bcl-2)were detected by Western blot.Results Compared with those induced with 5mmol/L glucose,HTR-8/SVneo cells induced with 25mmol/L glucose presented significantly lower cell survival rate,SOD level and protein expressions of PCNA and Bcl-2,but significantly higher apoptotic rate,MDA level and protein expressions of Notch1 and Bax(P＜0.05).Compared with those induced with 25mmol/L glucose,HTR-8/SVneo cells induced with 1.00mmol/L or 1.50mmol/L matrine presented significantly higher cell survival rate,SOD level and protein expressions of PCNA and Bcl-2,but significantly lower apoptotic rate,MDA level and protein expressions of Notch1 and Bax(P＜0.05).The treatment of Jagged1 significantly weakened the role of high-dose matrine in promoting the proliferation and inhibiting apoptosis of HTR-8/SVneo cells.Conclusion Matrine may improve the proliferation of high glucose-induced trophoblast cells and inhibit cell apoptosis by inhibiting the Notch signaling pathway.
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NSTL
万方数据
维普
