Impacts of evodiamine on proliferation and apoptosis of vascular smooth muscle cells in intracranial aneurysms by regulating the SDF-1α/CXCR4 signaling pathway
Objective To investigate the impacts of evodiamine on the proliferation and apoptosis of vascular smooth muscle cells(VSMCs)in intracranial aneurysms(IA)by regulating the stromal cell derived factor-1α(SDF-1α)/CXC chemokine receptor 4(CXCR4)signaling pathway.Methods Twenty four mice were randomly divided into control group and IA group.The IA model in mice was created by stereotactic injection of elastase,and the changes in the arterial tissue were observed by hematoxylin and eosin(H&E)staining.The effect of evodiamine at varying concentrations on the viability of mouse aortic vascular smooth muscle(MOVAS)cells was detected by MTT assay.Then,MOVAS cells were induced with blank control,H2O2,low-dose evodiamine(0.50μmol/L),high-dose evodiamine(1.00μmol/L)and high-dose evodiamine+CTCE-0214(1.00μmol/L evodiamine+10mg/kg SDF-1α/CXCR4 activator).Cell viability and apoptosis were detected by CCK-8 assay and flow cytometry,respectively.Western blot was applied to detect the protein expressions of SDF-1α,CXCR4,BAX,proliferating cell nuclear antigen(PCNA),smooth muscle 22α(SM22α)and α-smooth muscle actin(α-SMA).Results Compared with mice of blank control,IA mice showed significant pathological changes in the IA tissues and severe damage.Compared with those of blank control,MOVAS cells induced with H2O2 presented significantly higher apoptotic rate and protein expressions of SDF-1α,CXCR4 and BAX,and significantly lower cell survival rate and protein expressions of PCNA,SM22α and α-SMA(P＜0.05).Compared with those induced with H2O2,MOVAS cells induced with low,high-dose evodiamine presented significantly lower apoptotic rate and protein expressions of SDF-1α,CXCR4 and BAX,and significantly higher cell survival rate and protein expressions of PCNA,SM22α and α-SMA(P＜0.05).Compared with those induced with high-dose evodiamine,MOVAS cells induced with high-dose evodiamine+CTCE-0214 presented significantly higher apoptotic rate and protein expressions of SDF-1α,CXCR4 and BAX,and significantly lower cell survival rate and protein expressions of PCNA,SM22α and α-SMA(P＜0.05).Conclusion Evodiamine may inhibit the apoptosis and promote the proliferation of vascular smooth muscle cells in intracranial aneurysms by inhibiting the SDF-1α/CXCR4 signaling pathway.
NETL
NSTL
万方数据
维普
