Objective To investigate the impact of picroside Ⅱ(PⅡ)on the recovery of neural function in spinal cord injury(SCI)rats and its regulatory effect on the cyclic adenosine monophosphate(cAMP)-protein kinase A(PKA)signaling pathway.Methods The SCI rat model was established.Rats were randomly divided into control group(CT group),SCI group,low-dose PⅡ group(PⅡ L group,5 mg/kg/d),high-dose PⅡ group(PⅡ H group,20 mg/kg/d),and high-dose PⅡ+PKA inhibitor group(PⅡ H+H-89 group,20 mg/kg/d PⅡ+5 mg/kg/d H-89).The Basso,Beattie and Bresnahan(BBB)scoring was applied to evaluate the motor function of SCI rats.Hematoxylin and eosin(H&E)and Luxol fast blue(LFB)staining were performed to evaluate the pathological characteristics of spinal cord tissue and demyelination,respectively.Immunofluorescence was applied to detect the expressions of glial fibrillary acidic protein(GFAP)and ion calcium binding adapter molecule-1(IBA-1).Enzyme-linked immunosorbent assay(ELISA)was applied to measure relative levels of malondialdehyde(MDA),superoxide dismutase(SOD)and cyclic adenosine monophosphate(cAMP).Western blot was applied to detect the protein expressions of phosphorylated PKA(p-PKA),PKA,phosphorylated cyclic adenosine phosphate responsive component binding protein(p-CREB)and CREB.Results Compared with those the CT group,rats in SCI group had spinal cord tissue defects,cavities,infiltration of massive inflammatory cells,demyelination,significantly higher levels of GFAP,IBA-1 and MDA,but lower BBB,relative levels of SOD and cAMP,p-PKA/PKA and p-CREB/CREB(P<0.05).Compared with rats of SCI group,rats in the PⅡ L group and PⅡ H group had alleviated histological injuries,reduced cavities,inflammatory cells and demyelination,significantly lower levels of GFAP,IBA-1 and MDA,but higher BBB,relative levels of SOD and cAMP,p-PKA/PKA and p-CREB/CREB(P<0.05).Compared with rats of PⅡ H group,rats in PⅡ H+H-89 group had severer histological injuries and demyelination,significantly higher levels of GFAP,IBA-1 and MDA,but lower BBB,relative levels of SOD and cAMP,p-PKA/PKA and p-CREB/CREB(P<0.05).Conclusion PⅡ may promote the recovery of neurological function in SCI rats by activating the cAMP/PKA signaling pathway.
picroside Ⅱspinal cord injuryneurological functioncyclic adenosine monophosphate-protein kinase A
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