Effects of autologous platelet-rich plasma on inflammatory mediator levels,bone metabolism and the TLR4/TLR2/MyD88 signaling pathway in rats with scapulohumeral periarthritis
Effects of autologous platelet-rich plasma on inflammatory mediator levels,bone metabolism and the TLR4/TLR2/MyD88 signaling pathway in rats with scapulohumeral periarthritis
Objective To explore the effects of autologous platelet-rich plasma(PRP)on inflammatory mediators,bone metabolism and the TLR4/TLR2/MyD88 signaling patients in rats with scapulohumeral periarthritis(SP).Methods A total of 40 rats were randomly divided into control group,model group,PPP group and PRP group,with 10 rats in each group.Excluding the control group,rats in the other three groups were subjected to SP modeling.Injections of PPP and PRP for 2 weeks were performed in rats of PPP group and PRP group,respectively.Behavioral testing was assessed by the open field test.The levels of inflammatory factors in shoulder joint tissue and serum levels of bone metabolism indicators were determined by Enzyme-linked immunosorbent assay(ELISA).The shoulder joint morphology was observed by hematoxylin and eosin(H&E)staining.Protein expressions of key proteins involved in the TLR4/TLR2/MyD88 signaling pathway were determined by Western blotting.Results After interventions,rats in the model group,PPP group and PRP group presented significantly longer open field residence time and shorter 5-min walking distance than those of control group(P<0.05).There were no significant differences in the open field residence time and 5-min walking distance between model group and PPP group(P>0.05).Compared with those of model group and PPP group,rats in the PRP group presented significantly longer open field residence time and shorter 5-min walking distance(P<0.05).Compared with those of control group,rats in the model group,PPP group and PRP group presented significantly higher inflammatory mediator levels of TNF-α,IL-1β,PGE2,TLR4,TLR2 and MyD88 in the shoulder joint tissues,and higher serum levels of CTX-1,PINP and OC(P<0.05).Compared with those of model group and PPP group,rats in the PRP group presented significantly lower inflammatory mediator levels of TNF-α,IL-1β,PGE2,TLR4,TLR2 and MyD88 in the shoulder joint tissues,and lower serum levels of CTX-1,PINP and OC(P<0.05).Conclusion Autologous PRP can effectively reduce the levels of inflammatory mediators,balance bone metabolism and inhibit the protein expressions of TLR4,TLR2 and MyD88 in SP rats,which is worthy of further study and application.
关键词
自体富血小板血浆/肩周炎/炎性介质/骨代谢/Toll样受体
Key words
autologous platelet-rich plasma/periarthritis of shoulder/inflammatory mediators/bone metabolism/Toll-like receptor
维普
万方数据